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      QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices

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          Abstract

          A simple method for the determination of chloramphenicol in 22 matrices was prepared based on the QuEChERS and HPLC-MS/MS combination. Following a hydrolysis step, the homogenized samples were extracted and partitioned after adding sodium chloride with acetonitrile. Chloramphenicol was analysed by HPLC-MS/MS in negative electrospray mode by monitoring the daughter ions m/ z: 321→194 and 321→152. The limit of decision (CCα) was calculated at the range of 0.10 μg kg −1 to 0.15 μg kg −1 and detection capability (CCβ) from 0.12 μg kg −1 to 0.18 μg kg −1. Validation results showed that this method is suitable for the determination and confirmation of chloramphenicol in various matrices.

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          Determination of chloramphenicol residues in meat, seafood, egg, honey, milk, plasma and urine with liquid chromatography-tandem mass spectrometry, and the validation of the method based on 2002/657/EC.

          Elin Kristin Einarsen, Tone Normann Asp, Helene Rønning (2006)
          A simple and rapid method for the determination and confirmation of chloramphenicol in several food matrices with LC-MS/MS was developed. Following addition of d5-chloramphenicol as internal standard, meat, seafood, egg, honey and milk samples were extracted with acetonitrile. Chloroform was then added to remove water. After evaporation, the residues were reconstituted in methanol/water (3+4) before injection. The urine and plasma samples were after addition of internal standard applied to a Chem Elut extraction cartridge, eluted with ethyl acetate, and hexane washed. Also these samples were reconstituted in methanol/water (3+4) after evaporation. By using an MRM acquisition method in negative ionization mode, the transitions 321-->152, 321-->194 and 326-->157 were used for quantification, confirmation and internal standard, respectively. Quantification of chloramphenicol positive samples regardless of matrix could be achieved with a common water based calibration curve. The validation of the method was based on EU-decision 2002/657 and different ways of calculating CCalpha and CCbeta were evaluated. The common CCalpha and CCbeta for all matrices were 0.02 and 0.04 microg/kg for the 321-->152 ion transition, and 0.02 and 0.03 microg/kg for the 321-->194 ion transition. At fortification level 0.1 microg/kg the within-laboratory reproducibility is below 25%.
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            Chloramphenicol residues in chicken liver, kidney and muscle: a comparison among the antibacterial residues monitoring methods of Four Plate Test, ELISA and HPLC.

            H Tajik, H. Malekinejad, S.M. Razavi-Rouhani … (2010)
            There are many concerns about safety of food contaminated with antibacterial residues. This study was designed to investigate the occurrence of chloramphenicol (CAP) residue in broiler chickens tissues, namely liver, kidney and muscle. One hundred and sixty broiler chickens carcasses were collected from three provinces of Iran. Four Plate Test (FTP), ELISA and HPLC were used to qualify and quantify the contamination of the samples with CAP. The results of FPT revealed that up to 17.5% of the samples were contaminated with the antibiotic. The ELISA assay showed that out of 28 positive samples in FPT, 22 liver, 21 kidney and 14 muscle samples were positive for CAP. ELISA analyses demonstrated that the minimum and maximum levels of 0.54 and 155.2 ng/g were detected in the kidney and liver, respectively. HPLC analyses confirmed the ELISA findings although the level of contamination was lower than that of ELISA. These data showed that despite the prohibition of CAP application in food animals including poultry, the CAP residue was detectable indicating an illegal use of this antibiotic. Our findings also demonstrated the application of sensitive and more specific analytical assays in screening and quantitation of CAP residues in food products.
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              Determination of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in poultry, swine, bovine and fish by liquid chromatography-tandem mass spectrometry

              Fabiano Barreto, Cristina Ribeiro, Rodrigo Barcellos Hoff … (2016)
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                Author and article information

                Contributors
                Patricia Regal: Role: Academic Editor
                Journal
                Journal ID (nlm-ta): Molecules
                Journal ID (iso-abbrev): Molecules
                Journal ID (publisher-id): molecules
                Title: Molecules
                Publisher: MDPI
                ISSN (Electronic): 1420-3049
                Publication date (Electronic): 22 January 2019
                Publication date Collection: February 2019
                Volume: 24
                Issue: 3
                Electronic Location Identifier: 384
                Affiliations
                Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland; bartosz.sell@ 123456piwet.pulawy.pl (B.S.); marta.giergiel@ 123456piwet.pulawy.pl (M.G.); aposyn@ 123456piwet.pulawy.pl (A.P.)
                Author notes
                [* ]Correspondence: sniego@ 123456piwet.pulawy.pl ; Tel.: +48-818893144
                Author information
                https://orcid.org/0000-0003-3564-3279
                Article
                Publisher ID: molecules-24-00384
                DOI: 10.3390/molecules24030384
                PMC ID: 6384814
                PubMed ID: 30678224
                SO-VID: 04843ab1-6d02-48eb-b6b3-891d6857e87f
                Copyright © © 2019 by the authors.
                License:

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                Date received : 21 December 2018
                Date accepted : 22 January 2019
                Categories
                Subject: Article

                Keywords: chloramphenicol,multi matrices,confirmatory method,quechers,hplc–ms/ms
                Data availability:
                Keywords: chloramphenicol, multi matrices, confirmatory method, quechers, hplc–ms/ms

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