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      In vitro and in vivo antioxidant activities of inulin

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          Abstract

          This study was designed to investigate the in vitro and in vivo antioxidant activities of inulin. The in vitro assays demonstrated that the antioxidant activities of inulin, including the DPPH radical scavenging activity, ABTS scavenging activity and ferric reducing power, were weak and significantly lower than those of Vitamin C ( P < 0.05). The influence of dietary supplementation with inulin on the antioxidant status of laying hens was evaluated with in vivo antioxidant assays. The results indicated that inulin supplementation quadratically improved the egg production rate of the laying hens ( P < 0.01). The antioxidant enzyme activities in the serum, including SOD, CAT, and GSH-Px, and the total antioxidant capacity increased quadratically as inulin levels increased ( P < 0.001). The levels of MDA in the serum decreased quadratically as inulin levels increased ( P < 0.001). These findings suggest that inulin has the potential to improve the antioxidant status of laying hens.

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          Most cited references31

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          Antioxidant activity applying an improved ABTS radical cation decolorization assay.

          A method for the screening of antioxidant activity is reported as a decolorization assay applicable to both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants. The pre-formed radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS*+) is generated by oxidation of ABTS with potassium persulfate and is reduced in the presence of such hydrogen-donating antioxidants. The influences of both the concentration of antioxidant and duration of reaction on the inhibition of the radical cation absorption are taken into account when determining the antioxidant activity. This assay clearly improves the original TEAC assay (the ferryl myoglobin/ABTS assay) for the determination of antioxidant activity in a number of ways. First, the chemistry involves the direct generation of the ABTS radical monocation with no involvement of an intermediary radical. Second, it is a decolorization assay; thus the radical cation is pre-formed prior to addition of antioxidant test systems, rather than the generation of the radical taking place continually in the presence of the antioxidant. Hence the results obtained with the improved system may not always be directly comparable with those obtained using the original TEAC assay. Third, it is applicable to both aqueous and lipophilic systems.
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            A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates.

            1. A new method has been developed for measuring the total antioxidant capacity of body fluids and drug solutions, based on the absorbance of the ABTS.+ radical cation. 2. An automated method for use on a centrifugal analyser, as well as a manual method, is described. 3. The procedure has been applied to physiological antioxidant compounds and radical-scavenging drugs, and an antioxidant ranking was established based on their reactivity relative to a 1.0 mmol/l Trolox standard. 4. The Trolox equivalent antioxidant capacity of plasma from an adult reference population has been measured, and the method optimized and validated. 5. The method has been applied to investigate the total plasma antioxidant capacity of neonates and how this may be compromised in prematurity.
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              Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion

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                Author and article information

                Contributors
                Role: InvestigationRole: Writing – original draftRole: Writing – review & editing
                Role: ResourcesRole: Software
                Role: Data curationRole: Investigation
                Role: ResourcesRole: Supervision
                Role: ConceptualizationRole: MethodologyRole: Project administration
                Role: ConceptualizationRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS ONE
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                2 February 2018
                2018
                : 13
                : 2
                : e0192273
                Affiliations
                [1 ] College of Animal Science and Technology, Jilin Agricultural University, Jilin, Changchun, China
                [2 ] Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, Jilin Agricultural University, Jilin, Changchun, China
                [3 ] School of Life Sciences, Jilin Agricultural University, Jilin, Changchun, China
                [4 ] Grassland Research Institute of CAAS, Neimenggu, Hohhot, China
                Institute of medical research and medicinal plant studies, CAMEROON
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                http://orcid.org/0000-0003-2362-7984
                Article
                PONE-D-17-26649
                10.1371/journal.pone.0192273
                5796717
                29394273
                05a26eae-5c1a-4fa1-9eef-97975edf6d7e
                © 2018 Shang et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 16 July 2017
                : 26 December 2017
                Page count
                Figures: 3, Tables: 3, Pages: 12
                Funding
                Funded by: funder-id http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;
                Award ID: 31601972
                Award Recipient :
                Funded by: Project funded by China Postdoctoral Science Foundation
                Award ID: 2017M621224
                Award Recipient :
                This work was supported by the National Natural Science Foundation of China (31601972 to HMS) and Project funded by China Postdoctoral Science Foundation (2017M621224 to HMS).
                Categories
                Research Article
                Biology and Life Sciences
                Biochemistry
                Antioxidants
                Physical sciences
                Chemistry
                Chemical compounds
                Organic compounds
                Vitamins
                Vitamin C
                Physical sciences
                Chemistry
                Organic chemistry
                Organic compounds
                Vitamins
                Vitamin C
                Biology and Life Sciences
                Nutrition
                Diet
                Medicine and Health Sciences
                Nutrition
                Diet
                Physical Sciences
                Chemistry
                Chemical Radicals
                Free Radicals
                Physical Sciences
                Chemistry
                Physical Chemistry
                Chemical Radicals
                Free Radicals
                Biology and Life Sciences
                Biochemistry
                Enzymology
                Enzymes
                Dismutases
                Superoxide Dismutase
                Biology and Life Sciences
                Biochemistry
                Proteins
                Enzymes
                Dismutases
                Superoxide Dismutase
                Biology and Life Sciences
                Organisms
                Bacteria
                Gut Bacteria
                Bifidobacterium
                Biology and Life Sciences
                Agriculture
                Animal Management
                Animal Performance
                Research and Analysis Methods
                Bioassays and Physiological Analysis
                Biochemical Analysis
                Colorimetric Assays
                Custom metadata
                All relevant data are within the paper and its Supporting Information files.

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