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      Chlorins in mid-Cretaceous black shales of the Demerara Rise: The oldest known occurrence

      , , ,
      Organic Geochemistry
      Elsevier BV

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          Enhanced productivity led to increased organic carbon burial in the euxinic North Atlantic basin during the late Cenomanian oceanic anoxic event

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            Measurement of 13C and 15N isotopic composition on nanomolar quantities of C and N.

            We describe a trapping and chromatography system that cryogenically removes CO(2) and N(2) generated from sample combustion in an elemental analyzer (EA) and introduces these gases into a low-flow helium carrier stream for isotopic analysis. The sample size required for measurement by this system (termed nano-EA/IRMS) is almost 3 orders of magnitude less than conventional EA analyses and fills an important niche in the range of analytical isotopic methods. Only 25 nmol of N and 41 nmol of C are needed to achieve 1.0 per thousand precision (2sigma) from a single measurement while larger samples and replicate measurements provide better precision. Analyses of standards demonstrate that nano-EA measurements are both accurate and precise, even on nanomolar quantities of C and N. Conventional and nano-EA measurements on international and laboratory standards are indistinguishable within analytical precision. Likewise, nano-EA values for international standards do not differ statistically from their consensus values. Both observations indicate the nano-EA measurements are comparable to conventional EA analyses and accurately reproduce the VPDB and AIR isotopic scales. Critical to the success of the nano-EA system is the procedure for removing the blank contribution to the measured values. Statistical treatment of uncertainties for this procedure yields an accurate method for calculating internal and external precision.
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              Development and application of a high resolution liquid chromatographic method for the analysis of complex pigment distributions.

              Ternary and binary gradient systems have been developed for the high-performance liquid chromatographic analysis of complex pigment distributions typical of natural samples. Improved chromatographic resolution reveals significantly more pigment components in extracts from a sediment (Priest Pot, Cumbria, UK), a microbial mat (les Salines de la Trinital, South Catalonia, Spain) and a culture (C. phaeobacteroides) including novel bacteriochlorophyll derivatives. The methods developed are directly suited to LC-MS analysis and the automated acquisition of MS/MS data for pigments.
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                Author and article information

                Journal
                Organic Geochemistry
                Organic Geochemistry
                Elsevier BV
                01466380
                August 2011
                August 2011
                : 42
                : 7
                : 856-859
                Article
                10.1016/j.orggeochem.2011.04.002
                066a2053-7934-4945-970a-24a6f782a8c7
                © 2011

                https://www.elsevier.com/tdm/userlicense/1.0/

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