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      Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino Translated title: PGPB effect on the growth Pennisetum clandestinum under salt stress

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          Abstract

          Esta investigación tuvo como objetivo el aislamiento y caracterización de bacterias con potencial para promover el crecimiento del pasto Pennisetum clandestinum en suelos salinos simulados. De muestras de suelo rizosférico de P. clandestinum se aislaron 92 bacterias Gram positivas, diez aislados crecieron en agar nutritivo suplementado con NaCl (2,578 M). Los aislados se evaluaron bajo condiciones de invernadero; las cepas identificadas como KISA 34 y KISA 71 fueron seleccionadas como las mejores con base a la prueba estadística de Dunnet (p≤ 0,05) y fueron identificadas molecularmente como Bacillus amyloliquefaciens KISA 34 y Bacillus subtilis KISA 71. Estas cepas tienen la capacidad de producir; amonio, exopolisacáridos y celulosa, y tanto en presencia como ausencia de NaCl, produjeron índoles totales y solubilización de fósforo. La evaluación de las cepas en invernadero evidenció que el T6 - KISA 34 + KISA 71+ 25 % (Roca Fosfórica RF) con respecto al T2- testigo químico completo incrementaron de manera significativa la biomasa y el desarrollo de la planta (p≤ 0.05). Los resultados de esta investigación demostraron que las cepas aisladas tienen la capacidad de crecer en suelos salinos conservando sus características como promotoras de crecimiento vegetal con efectos positivos sobre P. clandestinum.

          Translated abstract

          This research aims the isolation and characterization of bacteria with potential to promote the growth of grass Pennisetum clandestinum in simulated saline soils. 92 Gram positive bacterias were isolated from Rhizosphere soil samples of P. clandestinum. Ten isolated bacteria grew on nutrient agar supplemented with NaCl (2.578 M). Isolates were evaluated under greenhouse conditions; the strains identified as KISA 34 and KISA 71 were selected as the best based on the statistical test of Dunnet (p ≤ 0.05) and were identified Bacillus amyloliquefaciens KISA 34 and Bacillus subtilis KISA 71. These strains have the ability to produce ammonium, exopolysaccharides and cellulose, both in absence and presence of NaCl. The strains produced indoles and phosphorus solubilization. The evaluation of strains in greenhouse showed that the T6 - KISA 34 + KISA 71+ 25 % (phosphate rock RF) significantly increased biomass and plant development (p≤ 0.05) compared with T2 - full fertilization . The results of this research showed that isolates have the ability to grow in saline soils retaining its characteristics as promoting plant growth with positive effects on P. clandestinum.

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          Most cited references43

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          Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen.

          The interaction of the direct dye Congo red with intact beta-D-glucans provides the basis for a rapid and sensitive assay system for bacterial strains possessing beta-(1 leads to 4),(1 leads to 3)-D-glucanohydrolase, beta-(1 leads to 4)-D-glucanohydrolase, and beta-(1 leads to 3)-D-glucanohydrolase activities. A close correspondence was observed between cellulolytic activity and beta-(1 leads to 4)-D-glucanohydrolase and beta-(1 leads to 4),(1 leads to 3)-D-glucanohydrolase activities in isolates from the bovine rumen. Many of these isolates also possessed beta-(1 leads to 3)-D-glucanohydrolase activity, and this characteristic may have taxonomic significance.
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            Exopolysaccharide-Producing Plant Growth-Promoting Rhizobacteria Under Salinity Condition

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              Isolation and characterization of PGPR and their effect on growth, yield and nutrient content in wheat (Triticum aestivum L.)

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                biote
                Revista Colombiana de Biotecnología
                Rev. colomb. biotecnol
                Instituto de Biotecnología, Universidad Nacional de Colombia
                0123-3475
                January 2016
                : 18
                : 1
                : 65-72
                Affiliations
                [1 ] Corporación Colombiana de Investigación Agropecuaria (Corpoica) Colombia
                [2 ] Corporación Colombiana de Investigación Agropecuaria (Corpoica) Colombia
                [3 ] Corporación Colombiana de Investigación Agropecuaria (Corpoica) Colombia
                Article
                S0123-34752016000100007
                10.15446/rev.colomb.biote.v18n1.50413
                072df99b-9c6c-4a53-97a2-23a7c98668ec

                This work is licensed under a Creative Commons Attribution 4.0 International License.

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                SciELO Colombia

                Self URI (journal page): http://www.scielo.org.co/scielo.php?script=sci_serial&pid=0123-3475&lng=en
                Categories
                BIOTECHNOLOGY & APPLIED MICROBIOLOGY

                Biotechnology
                Bacillus subtilis,Bacillus amyloliquefaciens,biofertilizantes,salinidad,solubilización de fósforo,biofertilizer,salinity,phosphorus solubilization

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