Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate

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Abstract

Due to the substantial genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV), commercial PRRS vaccines fail to provide sufficient cross protection. Previous studies have confirmed the existence of PRRSV broadly neutralizing antibodies (bnAbs). However, bnAbs are rarely induced by either natural infection or vaccination. In this study, we designed and synthesized a consensus sequence of PRRSV2 ORF2-6 genes (ORF2-6-CON) encoding all envelope proteins based on 30 representative Chinese PRRSV isolates. The ORF2-6-CON sequence shared > 90% nucleotide identities to all four lineages of PRRSV2 isolates in China. A chimeric virus (rJS-ORF2-6-CON) containing the ORF2-6-CON was generated using the avirulent HP-PRRSV2 JSTZ1712-12 infectious clone as a backbone. The rJS-ORF2-6-CON has similar replication efficiency as the backbone virus in vitro. Furthermore, pig inoculation and challenge studies showed that rJS-ORF2-6-CON is not pathogenic to piglets and confers better cross protection against the virulent NADC30-like isolate than a commercial HP-PRRS modified live virus (MLV) vaccine. Noticeably, the rJS-ORF2-6-CON strain could induce bnAbs while the MLV strain only induced homologous nAbs. In addition, the lineages of VDJ repertoires potentially associated with distinct nAbs were also characterized. Overall, our results demonstrate that rJS-ORF2-6-CON is a promising candidate for the development of a PRRS genetic engineered vaccine conferring cross protection.

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The online version contains supplementary material available at 10.1186/s13567-021-00944-8.

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Most cited references 64

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Clustal W and Clustal X version 2.0.

M.A. Larkin, G Blackshields, N.P. Brown … (2007)

The Clustal W and Clustal X multiple sequence alignment programs have been completely rewritten in C++. This will facilitate the further development of the alignment algorithms in the future and has allowed proper porting of the programs to the latest versions of Linux, Macintosh and Windows operating systems. The programs can be run on-line from the EBI web server: http://www.ebi.ac.uk/tools/clustalw2. The source code and executables for Windows, Linux and Macintosh computers are available from the EBI ftp site ftp://ftp.ebi.ac.uk/pub/software/clustalw2/

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Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Pathogenesis and Interaction with the Immune System.

Joan Lunney, Ying Fang, Andrea Ladinig … (2016)

This review addresses important issues of porcine reproductive and respiratory syndrome virus (PRRSV) infection, immunity, pathogenesis, and control. Worldwide, PRRS is the most economically important infectious disease of pigs. We highlight the latest information on viral genome structure, pathogenic mechanisms, and host immunity, with a special focus on immune factors that modulate PRRSV infections during the acute and chronic/persistent disease phases. We address genetic control of host resistance and probe effects of PRRSV infection on reproductive traits. A major goal is to identify cellular/viral targets and pathways for designing more effective vaccines and therapeutics. Based on progress in viral reverse genetics, host transcriptomics and genomics, and vaccinology and adjuvant technologies, we have identified new areas for PRRS control and prevention. Finally, we highlight the gaps in our knowledge base and the need for advanced molecular and immune tools to stimulate PRRS research and field applications.

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Molecular epidemiology of PRRSV: a phylogenetic perspective.

Mang Shi, Tommy Lam, Chung-Chau Hon … (2010)

Since its first discovery two decades ago, porcine reproductive and respiratory syndrome virus (PRRSV) has been the subject of intensive research due to its huge impact on the worldwide swine industry. Thanks to the phylogenetic analyses, much has been learned concerning the genetic diversity and evolution history of the virus. In this review, we focused on the evolutionary and epidemiological aspects of PRRSV from a phylogenetic perspective. We first described the diversity and transmission dynamics of Type 1 and 2 PRRSV, respectively. Then, we focused on the more ancient evolutionary history of PRRSV: the time of onset of all existing PRRSV and an origin hypothesis were discussed. Finally, we summarized the results from previous recombination studies to assess the potential impact of recombination on the virus epidemiology. Copyright © 2010 Elsevier B.V. All rights reserved.

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Author and article information

Contributors

Nanhua Chen:

ORCID: http://orcid.org/0000-0002-1909-8760

chnhlh@126.com

Shaobin Shang: shaobinshang@yzu.edu.cn

Jianzhong Zhu: jzzhu@yzu.edu.cn

Journal

Journal ID (nlm-ta): Vet Res

Journal ID (iso-abbrev): Vet Res

Title: Veterinary Research

Publisher: BioMed Central (London )

ISSN (Print): 0928-4249

ISSN (Electronic): 1297-9716

Publication date (Electronic): 27 May 2021

Publication date PMC-release: 27 May 2021

Publication date (Print): 2021

Volume: 52

Electronic Location Identifier: 74

Affiliations

[1 ]GRID grid.268415.c, College of Veterinary Medicine, , Yangzhou University, ; Yangzhou, 225009 Jiangsu China

[2 ]Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, 225009 Jiangsu China

[3 ]GRID grid.268415.c, Comparative Medicine Research Institute, , Yangzhou University, ; Yangzhou, 225009 Jiangsu China

[4 ]GRID grid.268415.c, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, ; Yangzhou, 225009 China

[5 ]National Research Center for Veterinary Medicine, Luoyang, 471003 Henan China

Author information

Nanhua Chen http://orcid.org/0000-0002-1909-8760

Article

Publisher ID: 944

DOI: 10.1186/s13567-021-00944-8

PMC ID: 8161975

PubMed ID: 34044890

SO-VID: 0801111c-5b9e-4e50-9966-01f29c49dfcd

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

History

Date received : 12 January 2021

Date accepted : 3 April 2021

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;

Award ID: 31802172

Award Recipient : Nanhua Chen

Funded by: FundRef http://dx.doi.org/10.13039/501100004608, Natural Science Foundation of Jiangsu Province;

Award ID: BK20170492

Award Recipient : Nanhua Chen

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ScienceOpen disciplines: Veterinary medicine

Keywords: prrsv,infectious clone,orf2-6 consensus sequence,broadly neutralizing antibodies,cross protection,genetic engineered vaccine

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ScienceOpen disciplines: Veterinary medicine

Keywords: prrsv, infectious clone, orf2-6 consensus sequence, broadly neutralizing antibodies, cross protection, genetic engineered vaccine

Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate

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Horses and Humans Research Foundation

Most cited references 64

Clustal W and Clustal X version 2.0.

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Pathogenesis and Interaction with the Immune System.

Molecular epidemiology of PRRSV: a phylogenetic perspective.

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