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      Gene Expression of Neurotrophins and Their High-Affinity Trk Receptors in Cultured Human Müller Cells

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          Abstract

          Purpose: To investigate the gene expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3) and their high-affinity receptors (TrkA, TrkB and TrkC) in cultured human Müller cells. Methods: The polymerase chain reaction was performed using specific primers for NGF, BDNF, NT-3, TrkA, TrkB and TrkC with complementary DNAs as the template, which were generated from poly-A+ RNA of cultured human Müller cells. Results: We detected the precursor mRNA transcripts for NGF, BDNF, NT-3, TrkB and TrkC, but not TrkA. Conclusion: Human Müller cells can direct mRNA expressions of a number of neurotrophins which may have a neurotrophic function in the retina. An autocrine mode of action is suggested, since Müller cells not only synthesize neurotrophic factors, but also express their specific receptors.

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          Astroglia—Neuron interactions that promote long-term neuronal survival

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            Author and article information

            Journal
            ORE
            Ophthalmic Res
            10.1159/issn.0030-3747
            Ophthalmic Research
            S. Karger AG
            0030-3747
            1423-0259
            2002
            February 2002
            07 February 2002
            : 34
            : 1
            : 38-42
            Affiliations
            aDepartment of Ophthalmology, Osaka Medical College, Osaka, and bDepartment of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
            Article
            48323 Ophthalmic Res 2002;34:38–42
            10.1159/000048323
            11834883
            10085df0-0f0a-40b2-b866-8e4c887192c8
            © 2002 S. Karger AG, Basel

            Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

            History
            Page count
            Figures: 3, Tables: 1, References: 23, Pages: 5
            Categories
            Original Paper

            Vision sciences,Ophthalmology & Optometry,Pathology
            Trk receptor,Polymerase chain reaction,Neurotrophin,Müller cell

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