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      Gene Expression of Neurotrophins and Their High-Affinity Trk Receptors in Cultured Human Müller Cells


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          Purpose: To investigate the gene expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3) and their high-affinity receptors (TrkA, TrkB and TrkC) in cultured human Müller cells. Methods: The polymerase chain reaction was performed using specific primers for NGF, BDNF, NT-3, TrkA, TrkB and TrkC with complementary DNAs as the template, which were generated from poly-A+ RNA of cultured human Müller cells. Results: We detected the precursor mRNA transcripts for NGF, BDNF, NT-3, TrkB and TrkC, but not TrkA. Conclusion: Human Müller cells can direct mRNA expressions of a number of neurotrophins which may have a neurotrophic function in the retina. An autocrine mode of action is suggested, since Müller cells not only synthesize neurotrophic factors, but also express their specific receptors.

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          Astroglia—Neuron interactions that promote long-term neuronal survival


            Author and article information

            Ophthalmic Res
            Ophthalmic Research
            S. Karger AG
            February 2002
            07 February 2002
            : 34
            : 1
            : 38-42
            aDepartment of Ophthalmology, Osaka Medical College, Osaka, and bDepartment of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
            48323 Ophthalmic Res 2002;34:38–42
            © 2002 S. Karger AG, Basel

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            Page count
            Figures: 3, Tables: 1, References: 23, Pages: 5
            Original Paper

            Vision sciences,Ophthalmology & Optometry,Pathology
            Trk receptor,Polymerase chain reaction,Neurotrophin,Müller cell


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