Neia Naldaiz-Gastesi 1 , 2 , 3 , María Goicoechea 2 , 3 , Sonia Alonso-Martín 4 , Ana Aiastui 2 , 3 , Macarena López-Mayorga 5 , Paula García-Belda 3 , 6 , Jaione Lacalle 1 , 2 , 7 , Carlos San José 8 , Marcos J. Araúzo-Bravo 9 , 10 , Lidwine Trouilh 11 , 12 , 13 , Véronique Anton-Leberre 11 , 12 , 13 , Diego Herrero 14 , Ander Matheu 10 , 15 , Antonio Bernad 14 , José Manuel García-Verdugo 3 , 6 , Jaime J. Carvajal 5 , Frédéric Relaix 4 , Adolfo Lopez de Munain 2 , 3 , 16 , 17 , Patricia García-Parra 1 , 2 , 3 , ∗ , Ander Izeta 1 , 18 , ∗∗
01 September 2016
The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5 + , Pax3/ Pax7 + cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment.
In this article, Izeta, García-Parra, and colleagues show that the panniculus carnosus (PC) muscle satellite cells originate from a somitic Pax3/7-positive and Myf5-positive lineage, like limb and body wall skeletal muscles. Through lineage tracing, cell sorting, and ablation experiments they unambiguously demonstrate that the only dermal cells with a myogenic potential are the PC satellite cells and their progeny.