ANÁLISE DA AÇÃO ANTIMICROBIANA DE CIMENTOS E PASTAS EMPREGADOS NA PRÁTICA ENDODÔNTICA

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Abstract

Foi avaliado o poder antimicrobiano dos cimentos Endomethasone, AH26, Sealer 26, Sealer 26 acrescido de 5% de hexametilenotetramina, Sealer 26 acrescido de 10% de hexametilenotetramina, Sealapex e pasta aquosa de hidróxido de cálcio. Foram utilizados o método de difusão radial em placas ágar escavadas e cepas puras de microorganismos, sendo que as leituras foram efetuadas após 24 e 48 horas de incubação em aerobiose e microaerofilia. Os resultados mostraram que o Endomethasone apresentou os maiores halos de inibição do crescimento bacteriano e que o acréscimo de hexametilenotetramina acarretou um aumento nos halos proporcionados pelo Sealer 26. O cimento Sealapex e a pasta de hidróxido de cálcio não inibiram os microorganismos testados

Translated abstract

An evaluation of the antimicrobial action of the sealers Endomethasone, AH26, Sealapex, Sealer 26, Sealer 26 with 5% hexametilenotetramine, Sealer 26 with 10% hexametilenotetramine and aqueous calcium hydroxide paste was carried out. Microorganisms were spread over hollowed agar plates, by means of the radial diffusion method. Halo measurements were done after 24 and 48 hours of incubation under aerobic and microaerophilia conditions. The results showed bigger halos when Endomethasone was used, and the increment of hexametilenotetramine in the Sealer 26 increased the inhibition halos of this sealer. The Sealapex and the calcium hydroxide paste did not inhibit bacterial growth

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Most cited references 51

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In vitro infection and disinfection of dentinal tubules.

Dag Ørstavik, M Haapasalo (1987)

An in vitro model for dentinal tubule infection of root canals was developed. Cylindrical dentin specimens, 4 mm high with a diameter of 6 mm and a canal 2.3 mm wide, were prepared from freshly extracted bovine incisors. The cementum was removed from all dentin blocks. The tubules were opened by four-minute treatments with 17% EDTA and 5.25% NaOCl before being infected with Enterococcus faecalis ATCC 29212 in yeast extract-glucose broth. Bacteria rapidly invaded the tubules. After three weeks of incubation, a heavy infection was found 400 micron from the canal lumen, and the front of the infection reached 1000 micron in some blocks. Camphorated paramonochlorophenol (CMCP) and a calcium hydroxide compound, Calasept, were tested for their disinfecting efficacy toward E. faecalis-infected dentin. Liquid CMCP rapidly and completely disinfected the dentinal tubules, whereas CMCP in gaseous form disinfected tubules less rapidly. Calasept failed to eliminate, even superficially, E. faecalis in the tubules. The method used in bacteriological sampling allowed for sequential removal of 100-micron-thick zones of dentin from the central canal toward the periphery. Control specimens were uniformly infected and yielded growth in bur samples up to some 500 microns from the surface. The model proved quite sensitive and seems suitable for in vitro testing of root canal medicaments.

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The antimicrobial activity of essential oils and essential oil components towards oral bacteria.

Frederick A. Meier, Joshua S. Shapiro, B Guggenheim (1994)

A method for reproducibly determining minimal inhibitory concentrations and minimal bactericidal concentrations of plant extracts towards fastidiously and facultatively anaerobic oral bacteria, predicated upon measurements of optical densities in microtitre plate wells, was devised. The antimicrobial properties of some botanical oils were surveyed; of these, Australian tea tree oil, peppermint oil, and sage oil proved to be the most potent essential oils, whereas thymol and eugenol were potent essential oil components.