- Record: found
- Abstract: not found
- Article: not found
Chemokine receptor CXCR2 regulates the functional properties of AMPA-type glutamate receptor GluR1 in HEK cells
August 2002
August 2002
There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
Experiments were conducted in both HEK cells and cerebellar neurons to investigate
whether CXC chemokine receptor 2 (CXCR2) is functionally coupled to GluR1. The co-expression
of CXCR2 with GluR1 in HEK cells increased (i) the GluR1 "apparent" affinity for the
transmitter; (ii) the GluR1 channel open probability; and (iii) GluR1 binding site
cooperativity upon CXCR2 stimulation with CXC chemokine ligand 2 (CXCL2). The affinity
of C-terminal-deleted GluR1 for glutamate (Glu) remained stable instead. Furthermore,
CXCL2 increased the binding site cooperativity of AMPA receptors in rat cerebellar
granule cells; and the amplitude of spontaneous excitatory postsynaptic current (sEPSCs)
in Purkinje neurons (PNs). Our findings indicate that the coupling of CXCR2 with GluR1
may modulate glutamatergic synaptic transmission.