Lanthanide chelate-encapsulated polystyrene nanoparticles for rapid and quantitative immunochromatographic assay of procalcitonin

Author(s): Tao Liao ¹ ^, ² ^, ³ ^, ⁴ ^, ⁵ , Fang Yuan ⁶ ^, ⁷ ^, ⁸ ^, ¹ ^, ² , Chuan Shi ¹ ^, ² ^, ³ ^, ⁴ ^, ⁵ , Chuan-Xin He ⁹ ^, ¹⁰ ^, ⁵ ^, ⁸ , Zigang Li ¹ ^, ² ^, ³ ^, ⁴ ^, ⁵

Publication date (Electronic): 2016

Journal: RSC Advances

Publisher: Royal Society of Chemistry (RSC)

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Abstract

Procalcitonin (PCT) is a potentially specific early marker of bloodstream infection and sepsis.

Abstract

Procalcitonin (PCT) is a potentially specific early marker of bloodstream infection and sepsis. In this work, we report, for the first time, the development of a fluorescence-based immunochromatographic strip test (IST) which employs Eu(DBM) ₃phen-containing nanoparticles as reporters for the detection of PCT in human serum. Experimental results demonstrate that the developed IST has an excellent ability for rapid (15 min), sensitive (0.05 ng mL ⁻¹) and quantitative analysis of PCT, and the assay results are comparable to which of the conventional enzyme-linked immunosorbent assay (ELISA). These results suggest that the developed assay could be utilized for point-of-care detection of PCT.

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Recent advances in the chemistry of lanthanide-doped upconversion nanocrystals.

Feng Wang, Xiaogang Liu (2009)

Lanthanide ions exhibit unique luminescent properties, including the ability to convert near infrared long-wavelength excitation radiation into shorter visible wavelengths through a process known as photon upconversion. In recent years lanthanide-doped upconversion nanocrystals have been developed as a new class of luminescent optical labels that have become promising alternatives to organic fluorophores and quantum dots for applications in biological assays and medical imaging. These techniques offer low autofluorescence background, large anti-Stokes shifts, sharp emission bandwidths, high resistance to photobleaching, and high penetration depth and temporal resolution. Such techniques also show potential for improving the selectivity and sensitivity of conventional methods. They also pave the way for high throughput screening and miniaturization. This tutorial review focuses on the recent development of various synthetic approaches and possibilities for chemical tuning of upconversion properties, as well as giving an overview of biological applications of these luminescent nanocrystals.

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Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey.

Geertruida Posthuma-Trumpie, Jakob Korf, Aart van Amerongen (2009)

Lateral flow (immuno)assays are currently used for qualitative, semiquantitative and to some extent quantitative monitoring in resource-poor or non-laboratory environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quantitative monitoring. We illustrate the potentials and limitations of analysis with lateral flow (immuno)assays using a literature survey and a SWOT analysis (acronym for "strengths, weaknesses, opportunities, threats"). Articles referred to in this survey were searched for on MEDLINE, Scopus and in references of reviewed papers. Search terms included "immunochromatography", "sol particle immunoassay", "lateral flow immunoassay" and "dipstick assay".