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Abstract
<p class="first" id="d4692994e93">A new adsorbent based on pH-responsive polymer assisted
aptamer functionalized magnetic
nanoparticles was developed for specific recognition and efficient adsorption of proteins.
Arising from the synergistic effect of specific affinity of apatamer on protein and
tunable hydrophobic/hydrophilic property of pH-responsive polymer, the adsorbent exhibited
excellent adsorption capacity for target protein. Notably, because of the pH-responsive
property of the polymer, the adsorption and desorption process could be regulated
through varying environmental pH. The resultant adsorbent that immobilized with lysozyme
binding aptamer was successfully applied in specific recognition and efficient adsorption
of lysozyme in egg white samples and good recovery results in the range of 95.2-103.2%
were obtained. Moreover, the adsorbent immobilized with cytochrome C binding aptamer
also exhibited satisfactory adsorption to cytochrome C. The synergistic effect of
pH-responsive polymer and aptamer promoted the recognition selectivity and adsorption
capacity to target protein, illustrating a facile way for construction of more specific
protein adsorbents.
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