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Abstract
Previous studies indicate that aspirin can promote neutrophil adhesion to venular
endothelium in vivo. The objectives of the present study were (1) to identify the
leukocyte and endothelial cell surface glycoproteins that mediate this adhesive interaction
and (2) to assess the role of lipoxygenase products, prostanoids, and platelet activating
factor in aspirin-induced neutrophil adhesion.
Human neutrophils (polymorphonuclear leukocytes [PMN]) were added to confluent monolayers
of human umbilical vein endothelial cells (HUVEC) and coincubated with or without
aspirin (30, 150, or 300 micrograms/mL).
Aspirin increased neutrophil adherence to HUVEC in a dose-dependent manner. Pretreatment
of HUVEC with aspirin had no effect on PMN adherence whereas pretreatment of PMN significantly
increased adherence to HUVEC. Incubation of neutrophils with aspirin increased surface
expression of CD11b and CD18 on neutrophils. The aspirin-induced increase in PMN adherence
to HUVEC was significantly reduced by monoclonal antibodies against CD18, CD11b, CD11a,
and intercellular adhesion molecule 1. Aspirin-induced neutrophil adhesion was diminished
by treatment with either a lipoxygenase inhibitor or a leukotriene B4 (LTB4) receptor
antagonist.
These studies indicate that aspirin promotes neutrophil adherence to endothelium via
CD11a/CD18- and CD11b/CD18-dependent interactions with intercellular adhesion molecule
1; the adhesion response is partially mediated by leukotriene B4.