Oral microbial profile in oral cancer patients before and after radiation therapy in a cancer care center – A prospective study

Abstract

Background:

Head and neck cancer is the sixth most common cancer reported worldwide. In many cases, the level of aggressiveness of therapy adopted in cancer patients may cause the alteration in oral microbiota; the emergence of potential pathogens may cause opportunistic infections in already immune-compromised individuals leading to increases in morbidity and mortality. Hence, this study was conducted to assess the oral microbial profile in oral cancer patients before and after radiotherapy.

Materials and Methods:

A total of 145 oral swabs were collected before radiotherapy ( n = 96), 3 months postradiotherapy ( n = 25), 6 months postradiotherapy ( n = 12) and controls ( n = 12). The samples were inoculated into brain–heart infusion broth and later in different media for bacterial isolation. The isolates were subjected to phenotypic characterization by automatic identification system.

Results:

Among the 96 samples studied from the preradiotherapy patient samples, Streptococcus species ( n = 28) were the predominant isolate, followed by Staphylococcus species ( n = 16), Enterobacter species ( n = 6) and Enterococcus species ( n = 6). Of the 25 samples studied 3 months after radiotherapy, Klebsiella pneumoniae ( n = 4) was isolated and 12 samples studied after 6 months of radiotherapy Candida species ( n = 4) and Pediococcus species ( n = 3) were isolated. Among the control group ( n = 12) screened, Streptococcus acidominimus ( n = 3) is the predominant bacteria isolated.

Conclusion:

High prevalence of Streptococcus sp. was found in patients of oral cancer before radiotherapy, while Candida albicans and Klebsiella species and Pediococcus species are the significant pathogens isolated in postradiotherapy cancer patients.