Primary root emission as a vigor test in soybean seeds

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Abstract

ABSTRACT The objective was to verify if the primary root emission can be recommended as a vigor test for soybean seeds. For this, five lots of varieties ANTA 82, NA7337 and BRS1074IPRO were evaluated by first count, germination, emergence, emergence speed index and tetrazolium tests. In the root protrusion test at 20, 25 and 30 ºC, two evaluation criteria were adopted: primary root emission precocity index (PREPI) and percentage of seeds that emitted the primary root (PRE). The seeds counted were those with a primary root equal to or greater than 2 mm, every 12 h from the installation of the test until 120 h. The design was completely randomized and means were compared, within each evaluation time, using the Scott-Knott test at 5% probability. Pearson’s linear correlation was performed for PREPI and PRE with emergence. PREPI was not considered efficient. For the PRE, at 20 ºC, there was a correlation at 72 h, for the three varieties. At 25 ºC there was a correlation for a period of 36 h for ANTA 82 and NA7337 and 60 h for BRS1074IPRO. At 30 ºC there was a correlation in the time of 36 h for the three cultivars. Considering that speed in obtaining results is important in vigor tests, it is concluded that primary root emission for 36 h after installing the test at 30 ºC is recommended as a vigor test in the quality control of soybean seeds.

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Seed vigor testing: an overview of the past, present and future perspective

Julio Marcos-Filho (2015)

The assessment of seed vigor has many important implications to the seed industry as a basic monitoring of seed physiological potential during different phases of seed production and a support for strategic decisions regarding the selection of high quality seedlots to meet the consumer demand. The potential attributes of seed vigor as a fundamental physiological seed characteristic and its association with field stand establishment and crop productivity has been worldwide recognized from the 1960s onward. This led to the diversification of research approaches involving the synchronization of different physiological characteristics and events that determine the potential for high performance during seed storage and after sowing. The basic objective of vigor testing is to provide a consistent identification of differences in physiological potential among seedlots of commercial value and this represents a more sensitive parameter than the germination test. There are various procedures to assess seed vigor including those that directly or indirectly evaluate seed metabolic state or identify seed tolerance to specific stress(es). At the same time, in more recent years, new knowledge has come from molecular biology, biotechnology, biophysics and seed and seedling imaging analyses; these approaches has been important complements to traditional seed research.. This text has the purpose to emphasize the role of Brazilian research in seed vigor, whose competence is internationally recognized, and to provide an overview of the evolution of knowledge also attempting to highlight events that contributed to the advancement of research on the subject.