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      Lippia javanica (Burm. F.) Herbal Tea: Modulation of Hepatoprotective Effects in Chang Liver Cells via Mitigation of Redox Imbalance and Modulation of Perturbed Metabolic Activities


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          Introduction: Hepatic oxidative injury is one of the pathological mechanisms that significantly contributes to the development of several liver diseases. In the present study, the hepatoprotective effect of Lippia javanica herbal tea was investigated in Fe 2+- mediated hepatic oxidative injury.

          Methods: Using an in vitro experimental approach, hepatic oxidative injury was induced by co-incubating 7 mM FeSO 4 with Chang liver cells that have been pre-incubated with or without different concentrations (15–240 μg/mL) of L. javanica infusion. Gallic acid and ascorbic acid served as the standard antioxidants.

          Results: The infusion displayed a reducing antioxidant activity in ferric-reducing antioxidant power (FRAP) assay and a potent scavenging activity on 2,2-diphenyl-2- picrylhydrazyl (DPPH) radical. Pretreatment with L. javanica infusion significantly elevated the levels of reduced glutathione and non-protein thiol, and the activities of superoxide dismutase (SOD) and catalase, with concomitant decrease in hepatic malondialdehyde levels, acetylcholinesterase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, glycogen phosphorylase and lipase activities. The infusion showed the presence of phytoconstituents such as phenolic compounds, tannins, phenolic glycosides and terpenoids when subjected to liquid chromatography—mass spectrometry analysis. Molecular docking revealed a strong binding affinity of dihydroroseoside and obacunone with both SOD and catalase compared to other phytoconstituents.

          Conclusion: These results portray a potent antioxidant and hepatoprotective effect of L. javanica, which may support the local usage of the herbal tea as a prospective therapeutic agent for oxidative stress-related liver diseases.

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          The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay.

          A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing "antioxidant power." Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration. Absorbance changes are linear over a wide concentration range with antioxidant mixtures, including plasma, and with solutions containing one antioxidant in purified form. There is no apparent interaction between antioxidants. Measured stoichiometric factors of Trolox, alpha-tocopherol, ascorbic acid, and uric acid are all 2.0; that of bilirubin is 4.0. Activity of albumin is very low. Within- and between-run CVs are <1.0 and <3.0%, respectively, at 100-1000 micromol/liter. FRAP values of fresh plasma of healthy Chinese adults: 612-1634 micromol/liter (mean, 1017; SD, 206; n = 141). The FRAP assay is inexpensive, reagents are simple to prepare, results are highly reproducible, and the procedure is straightforward and speedy. The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.
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            Tissue sulfhydryl groups

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              A new and rapid colorimetric determination of acetylcholinesterase activity


                Author and article information

                Front Pharmacol
                Front Pharmacol
                Front. Pharmacol.
                Frontiers in Pharmacology
                Frontiers Media S.A.
                07 August 2023
                : 14
                : 1221769
                [1] 1 Department of Pharmacology , University of the Free State , Bloemfontein, South Africa
                [2] 2 Department of Biochemistry , University of KwaZulu-Natal , Durban, South Africa
                [3] 3 Laser Research Centre , Faculty of Health Sciences , University of Johannesburg , Doornfontein, South Africa
                Author notes

                Edited by: Mansour Sobeh, Mohammed VI Polytechnic University, Morocco

                Reviewed by: Sultan Ayesh Mohammed Saghir, Al Hussein Bin Talal University, Jordan

                Oluwafemi Adeleke Ojo, Bowen University, Nigeria

                Rogers Mwakalukwa, Muhimbili University of Health and Allied Sciences, Tanzania

                *Correspondence: Motlalepula G. Matsabisa, matsabisamg@ 123456ufs.ac.za
                Copyright © 2023 Salau, Erukainure, Olofinsan, Schoeman and Matsabisa.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                : 12 May 2023
                : 27 July 2023
                Original Research
                Custom metadata

                Pharmacology & Pharmaceutical medicine
                oxidative stress,hepatotoxicity,gluconeogenesis,antioxidants,cholinergic enzyme


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