Analysis of Activity and Expression of the NLRP3, AIM2, and NLRC4 Inflammasome in Whole Blood.

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Abstract

Pro-inflammatory caspase-1 is a key player in innate immunity. Following activation in heterogenic protein complexes called the inflammasome , caspase-1 processes IL-1β and IL-18 to their mature forms and triggers pyroptosis. Here, we describe a small-volume whole blood assay facilitating the measurement of caspase-1 activity and inflammasome-related gene expression following specific stimulation of either the NLRP3, NLRC4, or AIM2 inflammasome .

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Journal

Journal ID (iso-abbrev): Methods Mol Biol

Title: Methods in molecular biology (Clifton, N.J.)

Publisher: Springer Science and Business Media LLC

ISSN (Electronic): 1940-6029

ISSN (Print): 1064-3745

Publication date (Electronic): 2022

Volume: 2459

Affiliations

[1 ] Department of Pediatrics, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.

[2 ] Department of Pediatrics, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany. stefan.winkler@uniklinikum-dresden.de.

Article

DOI: 10.1007/978-1-0716-2144-8_11

PubMed ID: 35212959

SO-VID: 20bfef2e-31f1-4dc0-9c6b-c69f0c0ff351

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Keywords: NLRP3,AIM2,Caspase-1,Inflammasome,MCC950,NLRC4,Whole blood assay

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Keywords: NLRP3, AIM2, Caspase-1, Inflammasome, MCC950, NLRC4, Whole blood assay

Analysis of Activity and Expression of the NLRP3, AIM2, and NLRC4 Inflammasome in Whole Blood.

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