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      Detection of oral streptococci in dental biofilm from caries-active and caries-free children Translated title: Detecção de estreptococos orais em biofilme dental de crianças cárie-ativas e livres de cárie

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          Abstract

          This work correlated the presence of oral streptococci in dental biofilm with clinical indexes of caries and oral hygiene in caries-active and caries-free children. S. mutans and/or S. sobrinus in the dental biofilm does not indicate a direct risk for developing dental caries.

          Translated abstract

          Este trabalho correlacionou a presença de estreptococos orais no biofilme dental com índices clínicos de cárie dentária e higiene oral em crianças com alta e baixa atividade de cárie. S. mutans e/ou S. sobrinus no biofilme dental não significa o imediato desenvolvimento de lesões cariosas.

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          Most cited references27

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          The Simplified Oral Hygiene Index

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            Role of Streptococcus mutans in human dental decay.

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              PCR detection and identification of oral streptococci in saliva samples using gtf genes.

              Oral streptococci are major constituents of dental plaque, and their prevalence is implicated in various pathologies. Therefore, accurate identification of oral streptococci would be valuable for studies of cariogenic plaque and for diagnostic use in infective endocarditis. Many oral streptococci possess glucosyltransferase enzymes that synthesize glucan, which is an obligate component of dental plaque. We established a rapid and precise method to identify oral streptococci by PCR using the species-specific region from the glucosyltransferase gene. With the species-specific primers, Streptococcus mutans, S. sobrinus, S. salivarius, S. sanguinis, S. oralis, and S. gordonii could be successfully distinguished. Further, we developed a simple method to extract the bacterial DNA from saliva. Using the resultant DNA as a template, the proposed PCR detection was performed. Their distribution was in accord with results of conventional biochemical tests. These findings indicate that the present PCR method is useful for the analysis of oral streptococci and can be successfully used in clinical applications to identify pathogenic bacteria associated with oral infectious disease and/or endocarditis.
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                Author and article information

                Journal
                bjm
                Brazilian Journal of Microbiology
                Braz. J. Microbiol.
                Sociedade Brasileira de Microbiologia (São Paulo, SP, Brazil )
                1517-8382
                1678-4405
                December 2008
                : 39
                : 4
                : 648-651
                Affiliations
                [03] Belo Horizonte MG orgnameUniversidade Federal de Minas Gerais orgdiv1Departamento de Bioquímica e Imunologia Brasil
                [02] PB orgnameUniversidade Federal da Paraíba orgdiv1Departamento de Biologia Molecular Brasil
                [01] João Pessoa PB orgnameUniversidade Federal da Paraíba orgdiv1Departamento de Odontologia Clínica e Social Brasil
                Article
                S1517-83822008000400009 S1517-8382(08)03900409
                220f99d8-2da2-4cd2-81b1-a37f1e48d517

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 25 February 2008
                : 23 October 2007
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 27, Pages: 4
                Product

                SciELO Brazil

                Self URI: Full text available only in PDF format (EN)
                Categories
                Medical Microbiology

                oral streptococci,glucosyltranferases,dental caries,cárie dentária,glicosiltransferases,PCR,estreptococos orais,biofilme dental,dental biofilm

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