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      Multifunctional yeast high-copy-number shuttle vectors.

      1 , , , ,
      Gene
      Elsevier BV

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          Abstract

          A set of four yeast shuttle vectors that incorporate sequences from the Saccharomyces cerevisiae 2 mu endogenous plasmid has been constructed. These yeast episomal plasmid (YEp)-type vectors (pRS420 series) differ only in their yeast selectable markers, HIS3, TRP1, LEU2 or URA3. The pRS420 plasmids are based on the backbone of a multifunctional phagemid, pBluescript II SK+, and share its useful properties for growth in Escherichia coli and manipulation in vitro. The pRS420 plasmids have a copy number of about 20 per cell, equivalent to that of YEp24. During non-selective yeast growth, pRS420 plasmids are lost through mitotic segregation at rates similar to other YEp vectors and yeast centromeric plasmid (YCp) vectors, in the range of 1.5-5% of progeny per doubling. The pRS420 series provides high-copy-number counterparts to the current pRS vectors [Sikorski and Hieter, Genetics 122 (1989) 19-27].

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          Author and article information

          Journal
          Gene
          Gene
          Elsevier BV
          0378-1119
          0378-1119
          Jan 02 1992
          : 110
          : 1
          Affiliations
          [1 ] Department of Microbiology, Southern Illinois University, Carbondale 62901.
          Article
          0378-1119(92)90454-W
          10.1016/0378-1119(92)90454-w
          1544568
          277d8b2d-f6f1-41db-90d7-7f50b37c939f
          History

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