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      Rediscovery of Crocus biflorus var. estriatus (Iridaceae) and its taxonomic characterisation

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      Italian Botanist

      Pensoft Publishers

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          Abstract

          The Italian endemic Crocusbiflorus usually shows white or lilac flowers with three-to-five striking violet longitudinal stripes on the outer tepals, but unstriped plants were recorded in the past. These plants were originally described as C.annulatussubvar.estriatus, and subsequently recombined as a variety of C.biflorus. The rediscovery of such plants in Toscana gave us the opportunity to clarify their systematic relationships, so that we typified the name, and performed karyological and ITS analyses. These plants share the same chromosome number (2 n = 2 x = 8) and ITS sequence with C.biflorus s. str.

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          Most cited references 8

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          International Code of Nomenclature for algae, fungi, and plants

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            An updated checklist of the vascular flora native to Italy

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              Direct amplification of the entire ITS region from poorly preserved plant material using recombinant PCR.

              Sequences of the internal transcribed spacers (ITS) of the nuclear ribosomal DNA are important molecular markers in phylogenetic analyses. To obtain sequences from herbarium material in which DNA often is severely degraded, the ITS region has to be amplified in two steps. Two methods that reduce bench time and reagents used are described. (i) Separately amplified preparations of subunits ITS-1 and ITS-2 are combined before purification. The presence of two fragments in the sequencing reaction does not impair the quality of sequences. (ii) Newly designed internal primers amplify partly overlapping regions of the two subunits. A combination of these internal primers with the external primers in one PCR allows the amplification of the entire ITS region even when degraded DNAs are used. This recombinant PCR approach, taking into account the +A bases added by several Taq DNA polymerases, will also be useful with other marker regions used in molecular phylogenetics.
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                Author and article information

                Journal
                Italian Botanist
                IB
                Pensoft Publishers
                2531-4033
                September 05 2018
                September 05 2018
                : 6
                : 23-30
                Article
                10.3897/italianbotanist.6.28729
                © 2018

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