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      Posible presencia de un gen R1 en germoplasma de Solanum tuberosum Grupo Phureja Translated title: Possible presence of a gene R1 in germplasm of Solanum tuberosum Phureja Group

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          Abstract

          Phytophthora infestans causa la gota, que es la enfermedad en la papa de mayor importancia económica a nivel mundial. La obtención de cultivares resistentes al patógeno es una estrategia necesaria para su control, por lo cual la identificación de genes de resistencia a P. infestans (Rpi) es fundamental para apoyar los programas de mejoramiento. La introgresión de Rpi desde especies silvestres o nativas dentro de cultivares es una vía para desarrollar cultivares con resistencia. S. phureja tiene notables características culinarias y nutricionales, e igualmente ha sido identificada como una fuente de resistencia a la gota, lo cual la sitúa en una posición de interés desde el punto de vista de recurso genético con fines de mejoramiento. Se caracterizaron genotípicamente 88 accesiones de S. phureja con marcadores moleculares tipo SCAR, el gen R1 de resistencia a P. infestans, el gen candidato StAOS2 y un marcador tipo CAPS ligados a loci para resistencia a P. infestans en Solanum tuberosum. Se presentó polimorfismo en los marcadores Prp1 y R1. La amplificación del marcador R1 mostró el alelo de 1.400 pb, característico del gen R1 en 17 accesiones de la colección de S. phureja, lo cual sugiere que estas accesiones posiblemente podrían tener un gen homólogo al gen R1 identificado en S. demissum

          Translated abstract

          Phytophthora infestans causes late blight in potato. This disease is the most destructive worldwide. Developing cultivars resistant to the pathogen is the most important strategy for its control, so identification of resistance genes (Rpi) is fundamental to support breeding programs. Introgression of Rpi from wild or native species within cultivars can be a good way to develop resistant cultivars. S. phureja has important culinary and nutrional traits and has been identified as a source of resistance to late blight. 88 accessions of S. phureja were genotypically characterized with molecular SCAR markers, the resistance gene R1 for P. infestans, the candidate gene StAOS2 and a CAPS marker linked to loci for resistance to P. infestans in Solanum tuberosum. Polymorphism was presented in Prp1 and R1 markers. The R1 marker amplification showed the 1,400 pb allele, which represents the R1 gene allele in 17 S. phureja accessions suggesting that these accessions could possibly have a homologous gene to the R1 gene identified in S. demissum

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          The Rpi-blb2 gene from Solanum bulbocastanum is an Mi-1 gene homolog conferring broad-spectrum late blight resistance in potato.

          The necessity to develop potato and tomato crops that possess durable resistance against the oomycete pathogen Phytophthora infestans is increasing as more virulent, crop-specialized and pesticide resistant strains of the pathogen are rapidly emerging. Here, we describe the positional cloning of the Solanum bulbocastanum-derived Rpi-blb2 gene, which even when present in a potato background confers broad-spectrum late blight resistance. The Rpi-blb2 locus was initially mapped in several tetraploid backcross populations, derived from highly resistant complex interspecific hybrids designated ABPT (an acronym of the four Solanum species involved:S. acaule, S. bulbocastanum, S. phureja and S. tuberosum), to the same region on chromosome 6 as the Mi-1 gene from tomato, which confers resistance to nematodes, aphids and white flies. Due to suppression of recombination in the tetraploid material, fine mapping was carried out in a diploid intraspecific S. bulbocastanum F1 population. Bacterial artificial chromosome (BAC) libraries, generated from a diploid ABPT-derived clone and from the resistant S. bulbocastanum parent clone, were screened with markers linked to resistance in order to generate a physical map of the Rpi-blb2 locus. Molecular analyses of both ABPT- and S. bulbocastanum-derived BAC clones spanning the Rpi-blb2 locus showed it to harbor at least 15 Mi-1 gene homologs (MiGHs). Of these, five were genetically determined to be candidates for Rpi-blb2. Complementation analyses showed that one ABPT- and one S. bulbocastanum-derived MiGH were able to complement the susceptible phenotype in both S. tuberosum and tomato. Sequence analyses of both genes showed them to be identical. The Rpi-blb2 protein shares 82% sequence identity to the Mi-1 protein. Significant expansion of the Rpi-blb2 locus compared to the Mi-1 locus indicates that intrachromosomal recombination or unequal crossing over has played an important role in the evolution of the Rpi-blb2 locus. The contrasting evolutionary dynamics of the Rpi-blb2/Mi-1 loci in the two related genomes may reflect the opposite evolutionary potentials of the interacting pathogens.
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            Assessing genetic potential in germplasm collections of crop plants by marker-trait association: a case study for potatoes with quantitative variation of resistance to late blight and maturity type

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              New R genes in Solanum demissum lindl. And their complementary races of Phytophthora infestans (Mont.) de bary

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                Author and article information

                Journal
                agc
                Agronomía Colombiana
                Agron. colomb.
                Universidad Nacional de Colombia, Facultad de Agronomía (Bogotá, Distrito Capital, Colombia )
                0120-9965
                August 2010
                : 28
                : 2
                : 137-146
                Affiliations
                [01] Bogotá orgnameUniversidad Nacional de Colombia orgdiv1Facultad de Agronomía orgdiv2Departamento de Agronomía Colombia tmosquerav@ 123456unal.edu.co
                Article
                S0120-99652010000200002 S0120-9965(10)02800202
                3d478a54-5592-49d2-944b-602c38985019

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 18 February 2010
                : 28 July 2010
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 52, Pages: 10
                Product

                SciELO Colombia

                Self URI: Texto completo solamente en formato PDF (ES)
                Categories
                Fitomejoramiento, recursos genéticos y biología molecular

                Solanum phureja,Phytophthora infestans,resistencia a gota,caracterización molecular,marcadores diagnóstico,late blight resistance,molecular characterization,diagnostic markers

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