Food and medicinal applications of barberry date back to 2500 years ago. This study investigates Berberis integerrima impact on lymphocytic immune responses.
Balb/c splenocytes were treated by 0.001–1000 μg/ml of B. integerrimaaqueous and alcoholic extracts in presence of phytohemagglutinin and lipopolysaccharide mitogens. Cell proliferation was assayed and cytokines were measured using ELISA.
Both extracts suppressed proliferation of phytohemagglutinin stimulated splenocytes (as T cells), while alcoholic extract induced expansion of lipopolysaccharide activated cells (as B lymphocytes) and unstimulated cells (p < 0.05). Both barberry extracts suppressed IFN-γ production (p < 0.05) and enhanced IL-4, IL-10 and TGF-β release from splenocytes (p < 0.05).
Food and medicinal applications of barberry date back to 2500 years. This study investigates Berberis integerrima's impact on lymphocytic immune responses. Balb/c splenocytes were treated by water and alcoholic extract of barberry which have changed cytokine release from cells. Both extracts could suppress T-cell expansion and enhance B-cell proliferation and also shift immune responses toward Th2. Therefore, B. integerrima contents can promote humoral immunity and would have beneficial applications in disease and conditions that humoral immunity needs to get stronger.