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      Different stocks of Prochilodus lineatus along the Cinzas River basin: contributions from a free-flowing tributary in the Capivara Reservoir region, Paranapanema River basin (upper Paraná River)

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          Abstract

          Abstract Dams are one of the main threats to Neotropical potamodromous fish, causing blockages of migratory routes and loss of breeding areas in an increasing number of drainage systems. In this scenario, current data argue that the maintenance of free-flowing tributaries along fragmented watersheds would be an important strategy to the migratory fish conservation, both in panmixia situations and in scenarios with different coexisting stocks, as already reported for Prochilodus lineatus. This study analyzed microsatellite loci and mitochondrial haplotypes in samples of P. lineatus obtained in different years along the Cinzas River basin, as well as in the Capivara Reservoir (largest Reservoir in the Paranapanema main channel, upper Paraná River) and in the Tibagi River, all connected by free stretches, aiming to investigate the contributions of the Cinzas River basin to the population dynamics of migratory fish in the Capivara reservoir region. Both markers detected high genetic diversity levels and indicated different genetic stocks of P. lineatus along the Cinzas River basin, suggesting some temporal genetic structuring, as well as the mixture of these stocks in the sample from the Capivara reservoir and from other points, corroborating the relationships among stocks in this reservoir and its tributaries.

          Translated abstract

          Resumo Barragens são umas das principais ameaças para peixes potamódromos Neotropicais, causando bloqueios de rotas migratórias e perdas de áreas de reprodução em um número crescente de sistemas de drenagem. Neste cenário, dados atuais defendem que a manutenção de tributários de fluxo livre em bacias hidrográficas fragmentadas seria uma importante estratégia na conservação de peixes migradores, tanto em situações de panmixia quanto em cenários com diferentes estoques coexistindo, tal como já relatado para Prochilodus lineatus. Este estudo analisou locos microssatélites e haplótipos mitocondriais em amostras de P. lineatus obtidas em diferentes anos ao longo da bacia do rio das Cinzas, no reservatório de Capivara (maior reservatório na calha principal do rio Paranapanema, alto rio Paraná) e no rio Tibagi, todos conectados por trechos livres, visando investigar as contribuições da bacia do rio das Cinzas na dinâmica populacional de peixes migradores na região do reservatório de Capivara. Ambos os marcadores detectaram altos níveis de diversidade genética e indicaram diferentes estoques de P. lineatus ao longo da bacia do rio das Cinzas, sugerindo alguma estruturação genética temporal, bem como a mistura destes estoques na amostra do reservatório de Capivara e de outros pontos, corroborando as relações de estoques neste reservatório e seus tributários.

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          Most cited references94

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          Detecting the number of clusters of individuals using the software structure: a simulation study

          The identification of genetically homogeneous groups of individuals is a long standing issue in population genetics. A recent Bayesian algorithm implemented in the software STRUCTURE allows the identification of such groups. However, the ability of this algorithm to detect the true number of clusters (K) in a sample of individuals when patterns of dispersal among populations are not homogeneous has not been tested. The goal of this study is to carry out such tests, using various dispersal scenarios from data generated with an individual-based model. We found that in most cases the estimated 'log probability of data' does not provide a correct estimation of the number of clusters, K. However, using an ad hoc statistic DeltaK based on the rate of change in the log probability of data between successive K values, we found that STRUCTURE accurately detects the uppermost hierarchical level of structure for the scenarios we tested. As might be expected, the results are sensitive to the type of genetic marker used (AFLP vs. microsatellite), the number of loci scored, the number of populations sampled, and the number of individuals typed in each sample.
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            STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method

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              DnaSP v5: a software for comprehensive analysis of DNA polymorphism data.

              DnaSP is a software package for a comprehensive analysis of DNA polymorphism data. Version 5 implements a number of new features and analytical methods allowing extensive DNA polymorphism analyses on large datasets. Among other features, the newly implemented methods allow for: (i) analyses on multiple data files; (ii) haplotype phasing; (iii) analyses on insertion/deletion polymorphism data; (iv) visualizing sliding window results integrated with available genome annotations in the UCSC browser. Freely available to academic users from: (http://www.ub.edu/dnasp).
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                Author and article information

                Journal
                ni
                Neotropical Ichthyology
                Neotrop. ichthyol.
                Sociedade Brasileira de Ictiologia (Maringá, PR, Brazil )
                1679-6225
                1982-0224
                2023
                : 21
                : 4
                : e230080
                Affiliations
                [2] Londrina Paraná orgnameInstituto Federal do Paraná orgdiv1Campus Londrina Brazil daniel.limeira@ 123456ifpr.edu.br
                [1] Cornélio Procópio orgnameUniversidade Estadual do Norte do Paraná orgdiv1Recursos Naturais e Limnologi orgdiv2Grupo de Pesquisas em Ecologia Brazil dhiego@ 123456uenp.edu.br
                [3] Londrina Paraná orgnameUniversidade Estadual de Londrina orgdiv1Laboratório de Genética e Ecologia Animal orgdiv2Departamento de Biologia Geral Brazil fernandasa@ 123456uel.br
                [4] Londrina Paraná orgnameUniversidade Estadual de Londrina orgdiv1Programa de Pós-Graduação em Ciências Biológicas orgdiv2Laboratório de Sistemática Molecular Brazil lenicesouza@ 123456hotmail.com
                Article
                S1679-62252023000400209 S1679-6225(23)02100400209
                10.1590/1982-0224-2023-0080
                407048cd-7232-496f-bfdf-4bdf06bdc9f6

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 25 October 2023
                : 15 July 2023
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 94, Pages: 0
                Product

                SciELO Brazil

                Categories
                Original Article

                D-Loop,Curimba,Potamodromous fish,Population genetics,Microsatellite,Peixe potamódromo,Microssatélites,Genética de populações

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