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      Qualitative and quantitative evaluation of Flos Puerariae by using chemical fingerprint in combination with chemometrics method

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          Abstract

          In order to better control the quality of Flos Puerariae (FP) , qualitative and quantitative analyses were initially performed by using chemical fingerprint and chemometrics methods in this study. First, the fingerprint of FP was developed by HPLC and the chemical markers were screened out by similarity analysis (SA), hierarchical clustering analysis (HCA), principal components analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA). Next, the chemical constituents in FP were profiled and identified by HPLC coupled to Fourier transform ion cyclotron resonance mass spectrometry (HPLC-FT-ICR MS). Then, the characteristic constituents in FP were quantitatively analyzed by HPLC. As a result, 31 common peaks were assigned in the fingerprint and 6 of them were considered as qualitative markers. A total of 35 chemical constituents were detected by HPLC-FT-ICR MS and 16 of them were unambiguously identified by comparing retention time, UV absorption wavelength, accurate mass, and MS/MS data with those of reference standards. Subsequently, the contents of glycitin, genistin, tectoridin, glycitein, genistein, and tectorigenin in 13 batches of FP were detected, ranging from 0.4438 to 11.06 mg/g, 0.955 to 1.726 mg/g, 9.81 to 57.22 mg/g, 3.349 to 41.60 mg/g, 0.3576 to 0.989 mg/g, and 2.126 to 9.99 mg/g, respectively. In conclusion, fingerprint analysis in combination with chemometrics methods could discover chemical markers for improving the quality control standard of FP. It is expected that the strategy applied in this study will be valuable for further quality control of other traditional Chinese medicines.

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          Highlights

          • •

            The qualitative and quantitative analysis were carried out for Flos Puerariae.

          • •

            Fingerprints were combined with different chemometrics to discover the qualitative markers FP.

          • •

            Thirty five constituents of FP were characterized by HPLC-FT-ICR MS.

          • •

            Six chemical constituents were simultaneously determined by HPLC.

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          Kudzu root: an ancient Chinese source of modern antidipsotropic agents.

          Wing Ming Keung, Bert L. Vallee (1998)
          Kudzu (Pueraria lobata) is one of the earliest medicinal plants used in traditional Chinese medicine. It has many profound pharmacological actions including antidipsotropic (antialcohol abuse) activity. Although both the roots and flowers of kudzu, Radix and Flos puerariae, respectively, have been used to treat alcohol abuse safely and effectively in China for more than a millennium, their true efficacy, active constituents, sites and mechanisms of action have never been critically examined. Recently, we have demonstrated that a crude extract of Radix puerariae suppresses the free-choice ethanol intake of ethanol-preferring golden Syrian hamsters and have identified two of its isoflavones, daidzin and daidzein, that account for this effect. Since then, we and other investigators have confirmed these findings in rats that were either trained or genetically bred to prefer and consume large amounts of ethanol. This article summarizes recent progress on the pharmacological and biochemical studies of the antidipsotropic isoflavones isolated from Radix puerariae.
          Article 0 comments Cited 37 times – based on 0 reviews     Review now
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            Saffron authentication based on liquid chromatography high resolution tandem mass spectrometry and multivariate data analysis.

            Josep Rubert, Ondrej Lacina, Milena Zachariasova … (2016)
            Saffron is one of the oldest and most expensive spices, which is often target of fraudulent activities. In this research, a new strategy of saffron authentication based on metabolic fingerprinting was developed. In the first phase, a solid liquid extraction procedure was optimized, the main aim was to isolate as maximal representation of small molecules contained in saffron as possible. In the second step, a detection method based on liquid chromatography coupled with high-resolution mass spectrometry was developed. Initially, principal component analysis (PCA) revealed clear differences between saffron cultivated and packaged in Spain, protected designation of origin (PDO), and saffron packaged in Spain of unknown origin, labeled Spanish saffron. Afterwards, orthogonal partial least square discriminant analysis (OPLS-DA) was favorably used to discriminate between Spanish saffron. The tentative identification of markers showed glycerophospholipids and their oxidized lipids were significant markers according to their origin.
            Article 0 comments Cited 28 times – based on 0 reviews     Review now
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              Chromatographic fingerprinting and related chemometric techniques for quality control of traditional Chinese medicines.

              Yizeng Liang, Peishan Xie, Footim Chau (2010)
              Development of chromatographic fingerprint (CF) and related chemometric methods and their applications to quality control of traditional Chinese medicines (TCMs) were discussed. CF is essentially a kind of quality control method for TCMs (or Chinese herbal medicines). Also, it is a quality-relevant-data high-throughput and integral tool to explore chemically the complexity of TCMs. With the help of chemometrics, some difficulties in evaluation and analysis of CFs, such as calculation of information content, peak alignment, pattern analysis, deconvolution of overlapping peaks, etc. could be well solved. To further explore TCMs synergic quality, intensive study of CF coupled with chemometrics will create the possibility to achieve the aim to reveal the working mechanisms of TCMs and to further control and strengthen TCMs' intrinsic quality in a comprehensive manner.
              Article 0 comments Cited 26 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Fei Han
                Journal
                Journal ID (nlm-ta): J Pharm Anal
                Journal ID (iso-abbrev): J Pharm Anal
                Title: Journal of Pharmaceutical Analysis
                Publisher: Xi'an Jiaotong University
                ISSN (Print): 2095-1779
                ISSN (Electronic): 2214-0883
                Publication date PMC-release: 25 September 2021
                Publication date (Print): June 2022
                Publication date (Electronic): 25 September 2021
                Volume: 12
                Issue: 3
                Pages: 489-499
                Affiliations
                [a ]School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China
                [b ]Department of Ophthalmology, the Fourth People's Hospital of Shenyang, Shenyang, 110031, China
                [c ]Science and Technology Institute of Shenyang Open University, Shenyang, 110003, China
                [d ]Shenyang Harmony Health Medical Laboratory, Shenyang, 110016, China
                Author notes
                [∗ ]Corresponding author. hanfei_spu@ 123456163.com
                [1]

                Both authors contributed equally to this work.

                Article
                Publisher Item ID: S2095-1779(21)00104-0
                DOI: 10.1016/j.jpha.2021.09.003
                PMC ID: 9257449
                PubMed ID: 35811625
                SO-VID: 42415a53-58ef-42b5-ba07-6eb9895b31d6
                Copyright © © 2021 The Authors
                License:

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                Date received : 23 March 2021
                Date revision received : 29 August 2021
                Date accepted : 11 September 2021
                Categories
                Subject: Original Article

                Keywords: flos puerariae,fingerprint analysis,chemometrics,hplc-ft-icr ms
                Data availability:
                Keywords: flos puerariae, fingerprint analysis, chemometrics, hplc-ft-icr ms

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