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      Presence of High Affinity Dopamine Receptors in Estrone-Induced, Prolactin-Secreting Rat Pituitary Adenomas: A Model for Human Prolactinomas

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          Abstract

          Adenomatous cells obtained from a pituitary tumor induced in Fisher 344/Lis rats by the subcutaneous implantation of estrone (E<sub>1</sub>) were found to secrete large amounts of prolactin (PRL). The secretion of PRL was stimulated by thyrotropin-releasing hormone (TRH) and low concentrations of dopamine (DA), while micromolar concentrations of DA were inhibitory. High affinity binding sites for <sup>3</sup>H-spiroperidol (<sup>3</sup>H-SPIR) were found to be present on the cells and to conform to the criteria of dopaminergic receptors. An adenylate cyclase (AC) present in the cells could be activated by a guanyl nucleotide and was inhibited by DA in the presence of guanosine 5´-triphosphate (GTP). Fractionation of the adenomatous cells by Percoll gradients identified two groups of cells capable of secreting PRL and bearing <sup>3</sup>H-SPIR binding sites. These data indicate that this rat pituitary adenoma may be a model for human prolactinomas that might be utilized for the study of the mechanism of action of dopaminergic drugs.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1985
          1985
          26 November 2008
          : 21
          : 2
          : 107-116
          Affiliations
          aNeuroendocrine Research Laboratory, Pediatric Research Center, Ste Justine Hospital and bClinical Research Institute, University of Montreal, Que., Canada
          Article
          180034 Horm Res 1985;21:107–116
          10.1159/000180034
          3920132
          © 1985 S. Karger AG, Basel

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          Pages: 10
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