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      Caracterización hematológica de Astronotus ocellatus (Cichliformes: Cichlidae): especie de importancia económica en la Amazonía peruana Translated title: Hematological characterization of Astronotus ocellatus (Cichliformes: Cichlidae): species of economic importance in the Peruvian Amazonia

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          Abstract

          RESUMEN El estudio tuvo como objetivo caracterizar los parámetros hematológicos de Astronotus ocellatus y determinar posibles correlaciones entre variables. Se colectaron muestras de sangre de 15 especímenes (peso: 109.43 ± 22.9 g; longitud total: 15.43 ± 1.28 cm), mantenidos en tanques de concreto. Los peces recibieron una alimentación con dieta comercial extruida, que contenía 42% de proteína bruta. La extracción de sangre se realizó mediante punción de la vena caudal usando jeringas con EDTA (10%). Los valo- res obtenidos de la serie eritrocítica fueron: hematocrito 24.5 ± 1.2%, hemoglobina 8.8 ± 2.4 g/dl, eritrocitos totales 1.10 ± 0.10 x106/µl, VCM 222.1 ± 41.0 fl, y CHCM 36.7 ± 10.4 g/ dl. En la serie leucocítica se identificaron: leucocitos totales 7.3 ± 2.6 x103/µl, linfocitos 67.3 ± 7.6%, monocitos 4.13 ± 1.4%, eosinófilos 27.5 ± 8.9% y neutrófilos 1.08 ± 1.3%. Para bioquímica sérica fueron: proteína total 3.44 ± 1.1 g/dl, glucosa 64.39 ± 28.8 mg/dl y colesterol 253.4 ± 91.2 mg/dl. Se registró correlación positiva entre hemoglobina-CHCM y correlación negativa entre peso y longitud con número de eritrocitos totales. La mayoría de los valores encontrados están dentro del rango reportado para esta especie.

          Translated abstract

          ABSTRACT The aim of this study was to characterize the hematological parameters of Astronotus ocellatus and to determine possible correlations between variables. Blood samples were collected from 15 specimens (weight: 109.43 ± 22.9 g, total length: 15.43 ± 1.28 cm), kept in concrete tanks and fed with a commercial diet extruded at 42% crude protein. Blood samples were collected by puncture of the caudal vein using syringes with EDTA (10%). The erythrocytic values were: haematocrit 24.5 ± 1.2%, haemoglobin 8.8 ± 2.4 g/dl; erythrocytes 1.10 ± 0.10 x 106/µl, MCV 222.1 ± 41.0 fl, MCHC 36.7 ± 10.4 g/dl. Leukocyte parameters were total leukocytes 7.3 ± 2.6 x103/µl, lymphocytes 67.3 ± 7.6%, monocytes 4.13 ± 1.4%, eosinophils 27.5 ± 8.9%, neutrophils 1.08 ± 1.3%. In serum biochemistry: total protein 3.44 ± 1.1 g/dl, glucose 64.39 ± 28.8 mg/dl, cholesterol 253.4 ± 91.2 mg/dl. Positive correlation was observed between haemoglobin and MCHC. Also, negative correlation between bodyweight and body length with the number of erythrocytes. The values found are within the range reported for this fish species.

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          Most cited references37

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          A New Blood Diluent for Counting the Erythrocytes and Leucocytes of the Chicken

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            Reproducibility in the Hematology Laboratory: The Microhematocrit Determination

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              Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid).

              Tilapia are a commonly aquacultured fish yet little is known about their normal physiology and response to disease. In this study we determined the results of complete hematologic (n=40) and plasma biochemical profiles (n=63) in production tilapia (Oreochromis hybrids). The fish were raised in recirculating systems with a high stocking density (120 g/L), and were in the middle of a 15-month production cycle. Blood was analyzed using standard techniques, and reference intervals were determined using nonparametric methods. Non-production tilapia (n=15) from low-density tanks (4 g/L) also were sampled; the clinical chemistry results were compared to reference intervals from the fish raised in high-density tanks. Differences were noted in plasma protein, calcium and phosphorus concentrations, such that reference intervals for high-density production tilapia were not applicable to fish raised under different environmental and management conditions.
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                Author and article information

                Journal
                rivep
                Revista de Investigaciones Veterinarias del Perú
                Rev. investig. vet. Perú
                Universidad Nacional Mayor de San Marcos. Facultad de Medicina Veterinaria (Lima, , Peru )
                1609-9117
                April 2020
                : 31
                : 2
                : e17827
                Affiliations
                [3] Iquitos orgnameCentro de Investigación y Promoción Popular Perú
                [2] Iquitos orgnameInstituto de Investigaciones de la Amazonía Peruana-IIAP orgdiv1Programa para el Uso y Conservación del Agua y sus Recursos-AQUAREC Perú
                [1] AP Macapá orgnameUniversidade Federal do Amapá orgdiv1Programa de Pós-Graduação em Biodiversidade Tropical Brazil anaigonzalesf@ 123456gmail.com
                Article
                S1609-91172020000200006 S1609-9117(20)03100200006
                10.15381/rivep.v31i1.17827
                453e8444-ff3d-4a17-aba6-5ed1f9fd0c18

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 03 May 2019
                : 26 March 2020
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 37, Pages: 0
                Product

                SciELO Peru

                Categories
                Artículos primarios

                acarahuazu,hematología,hematology,correlation,correlación,acarahuazú

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