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      Métodos analíticos ultrasensíveis: lente térmica e técnicas correlatas Translated title: Ultrasensitive analytical methods: thermal lens and related techniques

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          Translated abstract

          Among the emergent laser based spectrometric methods, thermal lensing and other photothermal techniques present a great potential for solving a variety of problems in the fields of chemistry, physics and biology. Their main advantages are high concentration sensitivity, sensibility to physical-chemical properties of the medium, excellent spatial resolution and noninvasive characteristics. In this article, theoretical principles, main applications and practical hints as well as fundamental limitations of these techniques will be carefully described. It is hoped that this will give the reader a clear picture of this field of investigation as well as provide to the ones who are not specialists in the area, the necessary background to understand, implement and use photothermal techniques. In the final sections the development frontiers of photothermal spectrometry will be discussed.

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          Triblock Copolymer Syntheses of Mesoporous Silica with Periodic 50 to 300 Angstrom Pores

          D. Zhao (1998)
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            Local characterization of hindered Brownian motion by using digital video microscopy and 3D particle tracking

            In this article we present methods for measuring hindered Brownian motion in the confinement of complex 3D geometries using digital video microscopy. Here we discuss essential features of automated 3D particle tracking as well as diffusion data analysis. By introducing local mean squared displacement-vs-time curves, we are able to simultaneously measure the spatial dependence of diffusion coefficients, tracking accuracies and drift velocities. Such local measurements allow a more detailed and appropriate description of strongly heterogeneous systems as opposed to global measurements. Finite size effects of the tracking region on measuring mean squared displacements are also discussed. The use of these methods was crucial for the measurement of the diffusive behavior of spherical polystyrene particles (505 nm diameter) in a microfluidic chip. The particles explored an array of parallel channels with different cross sections as well as the bulk reservoirs. For this experiment we present the measurement of local tracking accuracies in all three axial directions as well as the diffusivity parallel to the channel axis while we observed no significant flow but purely Brownian motion. Finally, the presented algorithm is suitable also for tracking of fluorescently labeled particles and particles driven by an external force, e.g. electrokinetic or dielectrophoretic forces.
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              Circular Dichroism and the Conformational Analysis of Biomolecules

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                Author and article information

                Contributors
                Role: ND
                Journal
                qn
                Química Nova
                Quím. Nova
                Sociedade Brasileira de Química (São Paulo )
                1678-7064
                July 1999
                : 22
                : 4
                : 565-573
                Affiliations
                [1 ] Universidade de São Paulo Brazil
                Article
                S0100-40421999000400015
                10.1590/S0100-40421999000400015
                4599f799-5dde-4531-8361-eb17e88b734a

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0100-4042&lng=en
                Categories
                CHEMISTRY, MULTIDISCIPLINARY

                General chemistry
                thermal lens,photothermal spectrometry,chemical analysis
                General chemistry
                thermal lens, photothermal spectrometry, chemical analysis

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