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      Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers

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          Abstract

          To assess the genetic diversity and genetic structure parameters, nine populations of Oryza glumaepatula from the Amazon biome, four from the Pantanal biome, and one collected at Rio Xingu, Mato Grosso, totaling 14 populations and 333 individuals were studied with isozyme markers. Six loci were evaluated showing a moderate allozyme variability (A = 1.21, P = 20.7%, Ho = 0.005, He = 0.060). The populations from the Pantanal biome showed higher diversity levels than the Amazon biome. High genetic differentiation among the populations, expected for self-fertilizing species, was observed (F ST=0.763), with lower differentiation found among the Pantanal populations (F ST=0.501). The average apparent outcrossing rate was higher for the Pantanal populations (t a = 0.092) than for the Amazonian populations (t a = 0.003), while the average for the 14 populations was 0.047, in accordance with a self-fertilization mating system.

          Translated abstract

          Utilizando marcadores isoenzimáticos, foram avaliadas nove populações de Oryza glumaepatula originárias da Amazônia, quatro do bioma do Pantanal, e uma coletada no Rio Xingu, Mato Grosso, totalizando 14 populações e 333 indivíduos, com o objetivo de avaliar a diversidade genética e a estrutura genética dessas populações. Seis locos foram avaliados, mostrando variabilidade alozímica moderada (A = 1.21, P = 20.7%, Ho = 0.005, He = 0.060). As populações do bioma Pantanal apresentaram níveis de diversidade mais altos que as da Amazônia. Alta diferenciação genética entre populações, esperada para espécies autógamas, foi observada (F ST=0.763), com menor diferenciação encontrada entre populações do Pantanal (F ST=0.501). A taxa média de cruzamento aparente foi maior para as populações do Pantanal (t a = 0.092) que da Amazônia (t a = 0.003), enquanto que a taxa media para as 14 populações foi 0.047, em concordância com o sistema reprodutivo por autogamia.

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          Most cited references37

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          Estimation of average heterozygosity and genetic distance from a small number of individuals.

          M Nei (1978)
          The magnitudes of the systematic biases involved in sample heterozygosity and sample genetic distances are evaluated, and formulae for obtaining unbiased estimates of average heterozygosity and genetic distance are developed. It is also shown that the number of individuals to be used for estimating average heterozygosity can be very small if a large number of loci are studied and the average heterozygosity is low. The number of individuals to be used for estimating genetic distance can also be very small if the genetic distance is large and the average heterozygosity of the two species compared is low.
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            FSTAT (Version 1.2): A computer program to calculate F-statistics

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              Correlated Matings in the Partial Selfer Mimulus guttatus

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                babt
                Brazilian Archives of Biology and Technology
                Braz. arch. biol. technol.
                Instituto de Tecnologia do Paraná - Tecpar (Curitiba )
                1678-4324
                October 2008
                : 51
                : 5
                : 873-882
                Affiliations
                [1 ] Universidade de São Paulo Brazil
                [2 ] Universidade Federal do Pará Brazil
                Article
                S1516-89132008000500001
                10.1590/S1516-89132008000500001
                48a504d8-fb98-4101-b7af-0cc2b72fc031

                http://creativecommons.org/licenses/by/4.0/

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                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1516-8913&lng=en
                Categories
                BIOLOGY

                General life sciences
                Genetic diversity,isozymes,Oryza glumaepatula,outcrossing rate,populations
                General life sciences
                Genetic diversity, isozymes, Oryza glumaepatula, outcrossing rate, populations

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