Zinc and nitrogen synergistic act on root-to-shoot translocation and preferential distribution in rice

Graphical abstract

Highlights

• •

  Zn promoted translocation and distribution of N into leaves and brown rice.

• •

  Zn induced the expression levels of N transporter genes in both root and shoot.

• •

  Zn increased the N assimilation level in leaves.

• •

  N promoted translocation and distribution of Zn into leaves and brown rice.

• •

  N up-regulated the expression levels of Zn transporter genes in both root and shoot.

Abstract

Introduction

Multiple studies have shown strong relationships between different nutrients in plants, and the important role of N in Zn acquisition and translocation has been recognized.

Objectives

The aim of this study was to estimate the effect of Zn on N uptake, translocation, and distribution in rice as well as the corresponding molecular mechanisms. We also aimed to evaluate the impact of N on the Zn content in rice grains which is closely related to the Zn nutrition in humans with rice-based diets.

Methods

We conducted both field trials and hydroponic cultures of two rice cultivars to analyze the growth and yield, the uptake, translocation, and distribution of N and Zn, as well as the expression of N transport and assimilation genes, and the Zn transporter genes under different combined applications of N and Zn.

Results

Zn supply promoted the root-to-shoot translocation (12–70% increasing) and distribution of N into the leaves (19–49% increasing) and brown rice (6–9% increasing) and increased the rice biomass (by 14–35%) and yield (by 13–63%). Zn supply induced the expression of OsNRTs and OsAMTs in both roots and shoots, but repressed the expression of OsNiR2, OsGS1;2, and OsFd-GOGAT in roots, whereas it activated the expression of OsNiR2, OsGS1;1, OsGS2, and OsFd-GOGAT in the shoots. Moreover, the enzyme activities of nitrite reductase, nitrate reductase, and glutamine synthetase increased and the free NO ₃ ^– concentration decreased, but the soluble protein concentration increased significantly in the shoots after Zn supply. Synergistically, N significantly facilitated the root-to-shoot translocation (1.68–11.66 fold) and distribution of Zn into the leaves (1.68–6.37 fold) and brown rice (7–12% increasing) and upregulated the expression levels of Zn transporter genes in both the roots and shoots.

Conclusions

We propose a working model of the cross-talk between Zn and N in rice plants, which will aid in the appropriate combined application of Zn and N fertilizers in the field to improve both N utilization in plants and Zn nutrition in humans with rice-based diets.