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      ISOLAMENTO DE CORYNEBACTERIUM PSEUDOTUBERCULOSIS NO SÊMEN DE UM CARNEIRO NA REGIÃO CENTRAL DO RIO GRANDE DO SUL Translated title: ISOLATION OF CORYNEBACTERIUM PSEUDOTUBERCULOSIS FROM THE SEMEN OF A SHEEP IN THE CENTRAL REGION OF RIO GRANDE DO SUL, BRAZIL

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          Abstract

          RESUMO O presente trabalho descreve o isolamento do Corynebacterium pseudotuberculosis no sêmen de um carneiro da raça Suffolk proveniente do Município de São Martinho da Serra, RS. Ao exame físico constatou-se aumento de volume subcutâneo aderido à pele, junto à inserção do escroto, próximo ao plexo pampiniforme esquerdo. Realizou-se exame andrológico com coleta de sêmen e, posteriormente, punção no local da lesão, obtendo-se material viscoso de coloração amarelo esverdeado. As amostras coletadas (sêmen e material obtido da punção) foram enviadas ao LABAC (Laboratório de Bacteriologia Veterinária da Universidade Federal de Santa Maria) para realização de cultura bacteriana. Em ambas as amostras, foi isolado o cocobacilo Gram positivo denominado C. pseudotuberculosis.

          Translated abstract

          ABSTRACT The present paper describes the isolation of Corynebacterium pseudotuberculosis from a semen sample of a Suffolk ram from São Martinho da Serra County, state of Rio Grande do Sul, Brazil. Physical examination revealed a subcutaneous mass close to the insertion of the scrotum, more specifically around the left pampiniform plexus. A routine andrological exam with semen collection was performed. Also, the mass was aspirated yielding a viscous greenish material. Samples were sent to a bacteriology lab for culture and identification. Gram positive coccobacillus C. pseudotuberculosis was isolated from both samples.

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          Most cited references10

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          Corynebacterium pseudotuberculosis and its role in ovine caseous lymphadenitis.

          Caseous lymphadenitis (CLA) of sheep, caused by Corynebacterium pseudotuberculosis, has been a significant disease in the majority of sheep-rearing regions for over a century. Because of the chronic and often sub-clinical nature of the infection, it has proved difficult to control and prevalence is high in many parts of the world, which in turn leads to significant economic losses for farmers. This review describes the important characteristics of C. pseudotuberculosis and examines the pathogenesis and epidemiology of the infection in sheep. The review also discusses the immune response to infection and describes the methods that have been developed to control CLA, with particular emphasis on the use of vaccination and serological testing.
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            Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR.

            The present study was carried out to estimate the prevalence of caseous lymphadenitis (CL) in sheep and goats slaughtered at the local abattoir in Elazig province located in the east of Turkey, between September and December 2000. A total of 2046 sheep and 2262 goat carcasses were examined during the study period and 118 abscessed lymph nodes, 89 from sheep and 29 from goats, were collected. Corynebacterium spp. strains were isolated from 81.4% of the abscesses, giving an overall prevalence of 2.2%. The prevalence was 3.5 and 1.1% in sheep and goats, respectively. PCR on DNA extracted from 96 suspicious isolates, using a pair of Corynebacterium pseudotuberculosis-specific primers, was positive for 93. Although cross-reaction with C. ulcerans, a human/bovine species, was observed, the PCR assay used in this study may successfully be applied for the diagnosis of CL in goats and sheep as an alternative to conventional methods, owing to its advantages of specificity and speed.
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              Identification of macrophage induced genes of Corynebacterium pseudotuberculosis by differential fluorescence induction.

              Corynebacterium pseudotuberculosis is the etiological agent of the sheep disease caseous lymphadenitis. We have developed a promoter reporter system for this organism based on expression of the green fluorescent protein (gfp) gene from Aequorea victoria. A promoterless vector, pSM20, containing the gfp gene was constructed, and promoters were inserted upstream of the gfp gene. Upon transformation into C. pseudotuberculosis, fluorescence could be visualised by fluorescence microscopy, and relative promoter strength measured by flow cytometry. The usefulness of this system for measuring changes in gene expression was demonstrated by measuring fluorescence levels of heat shocked C. pseudotuberculosis carrying a dnaK promoter construct. Replication of C. pseudotuberculosis within J774 macrophages could be monitored by fluorescence microscopy. The establishment of the system allowed the use of differential fluorescence to identify genes that showed up-regulation following macrophage infection. Genes coding for a non-ribosomal peptide synthetase and the beta chain of a propionyl CoA carboxylase were identified as possessing promoters that demonstrated enhanced activity following macrophage infection by C. pseudotuberculosis.
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                Author and article information

                Journal
                aib
                Arquivos do Instituto Biológico
                Arq. Inst. Biol.
                Instituto Biológico (São Paulo, SP, Brazil )
                0020-3653
                1808-1657
                March 2010
                : 77
                : 1
                : 139-142
                Affiliations
                [01] Santa Maria Rio Grande do Sul orgnameUniversidade Federal de Santa Maria orgdiv1Centro de Ciências Rurais orgdiv2Departamento de Grandes Animais Brazil
                [02] Santa Maria Rio Grande do Sul orgnameUniversidade Federal de Santa Maria orgdiv1Departamento de Medicina Veterinária Preventiva Brazil
                Article
                S1808-16572010000100139 S1808-1657(10)07700100139
                10.1590/1808-1657v77p1392010
                4e9208d8-2362-4e02-92f8-c66fa414a6ff

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 08 April 2009
                : 09 October 2009
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 13, Pages: 4
                Product

                SciELO Brazil

                Categories
                Comunicação Científica

                caseous lymphadenitis,linfadenite caseosa,Corynebacterium pseudotuberculosis,ovinos,sêmen,sheep,semen

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