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ANATOMICAL STUDIES OF IN VITRO ORGANOGENESIS INDUCED IN LEAF-DERIVED EXPLANTS OF PASSIONFRUIT Translated title: ESTUDOS ANATÔMICOS DA ORGANOGÊNESE IN VITRO INDUZIDA EM EXPLANTES DE FOLHA DE MARACUJÁ

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      Abstract

      With the aim of studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of parenchymal origin.

      Translated abstract

      Com o objetivo de estudar a organogênese in vitro em Passiflora edulis Sims f. flavicarpa Deg., o maracujá-amarelo, explantes derivados de folha foram cultivados em meio contendo NAA ou BAP, no escuro e na presença de luz. Foram descritos os eventos histológicos que levam à formação de novo de órgãos. Concluiu-se que o escuro induz a rizogênese, na presença de NAA, enquanto a regeneração de brotos é estimulada pela luz e BAP. Esta condição é recomendada para micropropagar o maracujá uma vez que vários brotos adventícios são formados a partir de meristemóides de origem parenquimática.

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      Most cited references 28

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            Author and article information

            Affiliations
            [1 ] Universidade de São Paulo Brazil
            [2 ] Universidade de São Paulo Brazil
            [3 ] Universidade de Paris XI França
            Contributors
            Role: ND
            Role: ND
            Role: ND
            Journal
            pab
            Pesquisa Agropecuária Brasileira
            Pesq. agropec. bras.
            Embrapa Informação Tecnológica (Brasília )
            1678-3921
            November 1999
            : 34
            : 11
            : 2007-2013
            S0100-204X1999001100005
            10.1590/S0100-204X1999001100005

            http://creativecommons.org/licenses/by/4.0/

            Product
            Product Information: SciELO Brazil
            Categories
            AGRICULTURE, DAIRY & ANIMAL SCIENCE
            AGRICULTURE, MULTIDISCIPLINARY

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