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      ANATOMICAL STUDIES OF IN VITRO ORGANOGENESIS INDUCED IN LEAF-DERIVED EXPLANTS OF PASSIONFRUIT Translated title: ESTUDOS ANATÔMICOS DA ORGANOGÊNESE IN VITRO INDUZIDA EM EXPLANTES DE FOLHA DE MARACUJÁ

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          Abstract

          With the aim of studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of parenchymal origin.

          Translated abstract

          Com o objetivo de estudar a organogênese in vitro em Passiflora edulis Sims f. flavicarpa Deg., o maracujá-amarelo, explantes derivados de folha foram cultivados em meio contendo NAA ou BAP, no escuro e na presença de luz. Foram descritos os eventos histológicos que levam à formação de novo de órgãos. Concluiu-se que o escuro induz a rizogênese, na presença de NAA, enquanto a regeneração de brotos é estimulada pela luz e BAP. Esta condição é recomendada para micropropagar o maracujá uma vez que vários brotos adventícios são formados a partir de meristemóides de origem parenquimática.

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          Most cited references 28

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          Botanical microtechnique

           JE SASS,  J.E. SASS,  J. SASS (1958)
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            Die Nadel der Schwarzkiefer-Massenprodukt und Kunstwerk der Natur

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              Shoot Histogenesis in Cotyledon Explants of Radiata Pine

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                Author and article information

                Affiliations
                [1 ] Universidade de São Paulo Brazil
                [2 ] Universidade de São Paulo Brazil
                [3 ] Universidade de Paris XI França
                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                pab
                Pesquisa Agropecuária Brasileira
                Pesq. agropec. bras.
                Embrapa Informação Tecnológica (Brasília )
                1678-3921
                November 1999
                : 34
                : 11
                : 2007-2013
                S0100-204X1999001100005
                10.1590/S0100-204X1999001100005

                http://creativecommons.org/licenses/by/4.0/

                Product
                Product Information: SciELO Brazil
                Categories
                AGRICULTURE, DAIRY & ANIMAL SCIENCE
                AGRICULTURE, MULTIDISCIPLINARY

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