Characteristics of sperm motility in boar semen diluted in different extenders and stored for seven days at 18 degrees C.

Abstract

Although numerous extenders exist for diluting boar semen, little research has been conducted comparing commercial extenders with regard to maintaining sperm motility during storage. The objective was to use a computer- assisted sperm analysis system to assess motility of boar spermatozoa diluted in Beltsville Thawing Solution, Merck-III, Androhep-lite, Sperm Aid, MR-A, Modena, X-Cell, VSP, and Vital. Ejaculates from boars (n=10) were collected and sub-samples were diluted (35x10(6) spermatozoa/ml) in the different extenders and stored for seven days at 18 degrees. Extender by day interactions were detected (p<0.01) and on each day post collection, there were numerically small, but statistically significant differences in characteristics of sperm motility among extenders. For example, on day 7, the percentages of motile and progressively motile spermatozoa were highest (p<0.05) in X-Cell (90.7%) and Modena (63.9%), respectively. The average velocity measured over the actual point-to-point track followed by the sperm cell (VCL; 198.2 microm/s) and path velocity of the smoothed cell path (VAP; 106.4 microm/s) were highest (p<0.05) in Vital and Modena, respectively. Average velocity measured in a straight line from the beginning to the end of the track (VSL; 78.3 microm/s), average value of the ratio VSL/VAP (straightness; 73.2) and average value of the ratio VSL/VCL (linearity; 44.1) on day 7 were highest in Androhep-lite. In summary, changes in sperm motility during storage were affected by the extender utilized, but with the exception of Sperm Aid, all extenders maintained a high degree of sperm motility through 7 days of storage.