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Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique

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      Abstract

      In modern bioanalytical methods, it is often desired to detect several targets in one sample within one measurement. Immunological methods including those that use superparamagnetic beads are an important group of techniques for these applications. The goal of this work is to investigate the feasibility of simultaneously detecting different superparamagnetic beads acting as markers using the magnetic frequency mixing technique. The frequency of the magnetic excitation field is scanned while the lower driving frequency is kept constant. Due to the particles’ nonlinear magnetization, mixing frequencies are generated. To record their amplitude and phase information, a direct digitization of the pickup-coil’s signal with subsequent Fast Fourier Transformation is performed. By synchronizing both magnetic fields, a stable phase information is gained. In this research, it is shown that the amplitude of the dominant mixing component is proportional to the amount of superparamagnetic beads inside a sample. Additionally, it is shown that the phase does not show this behaviour. Excitation frequency scans of different bead types were performed, showing different phases, without correlation to their diverse amplitudes. Two commercially available beads were selected and a determination of their amount in a mixture is performed as a demonstration for multiplex measurements.

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          Detection of a single magnetic microbead using a miniaturized silicon Hall sensor

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            Author and article information

            Affiliations
            [1 ]Institute of Complex Systems Bioelectronics (ICS-8), Forschungszentrum Jülich, 52425 Jülich, Germany; s.achtsnicht@ 123456fz-juelich.de (S.A.); a.pourshahidi@ 123456fz-juelich.de (A.M.P.); a.offenhaeusser@ 123456fz-juelich.de (A.O.)
            [2 ]RWTH Aachen University, 52062 Aachen, Germany
            Author notes
            [* ]Correspondence: h.-j.krause@ 123456fz-juelich.de ; Tel.: +49-2461-61-2955
            Journal
            Sensors (Basel)
            Sensors (Basel)
            sensors
            Sensors (Basel, Switzerland)
            MDPI
            1424-8220
            07 June 2019
            June 2019
            : 19
            : 11
            31181672
            6603599
            10.3390/s19112599
            sensors-19-02599
            © 2019 by the authors.

            Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

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