Glycolipids and sphingolipids are well known for their diverse biological activities
like anticancer, anti-inflammatory, antistress, anti-HIV, hepatoprotective and antimicrobial.
The present study deals with the activity-guided isolation and characterization of
two antihyperglycemic glycolipids, (2S)-1,2-di-O-octadecanoyl-3-O-[α-d-galctopyranosyl-(1'' → 6')-O-β-d-galactopyranosyl]
glycerol (1) and 1-O-β-d-glucopyranosyl-(2S,3S,4R,8E)-2-[(2R)-2-hydroxy-tetracosanoylamino]-2,3,4-octadecanetriol-8-ene
(2) from Oplismenus burmannii and the development of a simple and validated reverse-phase
HPLC analytical method for their quantification in the methanolic extracts of O. burmannii.
The marker compounds 1 and 2 were isolated from the methanolic extract of O. burmannii
and characterized on the basis of their spectroscopic data. Their antihyperglycemic
potential was evaluated by determining their glucose uptake-stimulating potential
in L6-GLUT4myc myotube cells. Finally, these analytes were separated on a Waters Spherisorb
ODS 2 column with a binary gradient of methanol and water at a constant flow rate
of 0.8 mL/min and detected using a photodiode array detector at 230 nm. The calibration
curve was linear (r(2) > 0.999) over 1.2 orders of magnitude with acceptable accuracy,
reproducibility and recovery (98.16-100.50%). The limits of detection and quantification
for 1 and 2 were 1.36, 4.11 and 1.11, 3.35 µg/mL respectively. The method is simple,
accurate, precise and selective and may be routinely used for the quality control
analysis of whole plant extract of O. burmannii for these two glycolipids.