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      • Record: found
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      • Article: found

      Water-Soluble and Insoluble Crystallins of the Developing Human Fetal Lens, Analyzed by Agarose/Polyacrylamide Thin-Layer Isoelectric Focusing

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          Abstract

          Eight human fetal lenses, selected on basis of normality, of a gestational age of 119 to 231 days were analyzed by thin-layer isoelectric focusing (IEF) in agarose/polyacrylamide gels. This method was adapted for the separation of lens crystallins into HM-, α-, β-and γ-crystallins. It is especially suitable for analysis under non-denaturing conditions of high-molecular-weight crystallins and of insoluble crystallins (WI) solubilized in formamide. The latter could be separated into HM-, α- and γ-crystallins. During fetal development, a considerable increase of γ-crystallin proportion was observed due to new synthesis. This increase was balanced by a decrease of α-crystallin proportion.

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          Author and article information

          Journal
          Journal ID (publisher-id): ORE
          Journal ID (nlm-ta): Ophthalmic Res
          Journal ID (doi): 10.1159/issn.0030-3747
          Title: Ophthalmic Research
          Publisher: S. Karger AG
          ISSN (Print): 0030-3747
          ISSN (Electronic): 1423-0259
          Publication date Subscription-year: 1987
          Publication date (Print): 1987
          Publication date (Electronic): 04 December 2009
          Volume: 19
          Issue: 3
          Pages: 150-156
          Affiliations
          aDepartment of Experimental Ophthalmology, and bInstitute of Pediatric Pathology, University of Bonn, FRG
          Article
          Publisher ID: 265487 Other ID: Ophthalmic Res 1987;19:150–156
          DOI: 10.1159/000265487
          PubMed ID: 3658325
          SO-VID: 63c4fe8d-09c2-4577-a15c-e0dba7e893e4
          Copyright © © 1987 S. Karger AG, Basel
          License:

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Page count
          Pages: 7
          Categories
          Subject: Original Paper

          ScienceOpen disciplines: Vision sciences,Ophthalmology & Optometry,Pathology
          Keywords: Water-soluble crystallins,Prenatal lens development,Water-insoluble crystallins,HM-, α-, β- and γ-crystallins,Agarose/polyacrylamide isoelectric focusing
          Data availability:
          ScienceOpen disciplines: Vision sciences, Ophthalmology & Optometry, Pathology
          Keywords: Water-soluble crystallins, Prenatal lens development, Water-insoluble crystallins, HM-, α-, β- and γ-crystallins, Agarose/polyacrylamide isoelectric focusing

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