Optimization of H2SO4-catalyzed hydrothermal pretreatment of rapeseed straw for bioconversion to ethanol: Focusing on pretreatment at high solids content
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Abstract
A central composite design of response surface method was used to optimize H(2)SO(4)-catalyzed
hydrothermal pretreatment of rapeseed straw, in respect to acid concentration (0.5-2%),
treatment time (5-20 min) and solid content (10-20%) at 180 degrees C. Enzymatic hydrolysis
and fermentation were also measured to evaluate the optimal pretreatment conditions
for maximizing ethanol production. The results showed that acid concentration and
treatment time were more significant than solid content for optimization of xylose
release and cellulose recovery. Pretreatment with 1% sulfuric acid and 20% solid content
for 10 min at 180 degrees C was found to be the most optimal condition for pretreatment
of rapeseed straw for ethanol production. After pretreatment at the optimal condition
and enzymatic hydrolysis, 75.12% total xylan and 63.17% total glucan were converted
to xylose and glucose, respectively. Finally, 66.79% of theoretical ethanol yielded
after fermentation.