For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
32
views
23
references
 Top references
cited by
3
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
1 collections
    0
    shares
      645
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Optimization of Protein Extraction for Lichen Thalli

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study.

          Related collections

          Most cited references23

          • Record: found
          • Abstract: found
          • Article: not found

          Biopharmaceutical potential of lichens.

          P. Christopher, Vasudeo Zambare (2012)
          Lichens are composite organisms consisting of a symbiotic association of a fungus (the mycobiont) with a photosynthetic partner (the phytobiont), usually either a green alga or cyanobacterium. The morphology, physiology and biochemistry of lichens are very different from those of the isolated fungus and alga in culture. Lichens occur in some of the most extreme environments on the Earth and may be useful to scientists in many commercial applications. Over the past 2 decades, there has been a renewed and growing interest in lichens as a source of novel, pharmacologically active biomolecules. This review summarizes the past and current research and development trends in the characterization and use of lichens and their bioactive compounds in traditional medicine and other biopharmaceutical applications of commercial interest. The present review contains 10 illustrations and 188 references compiled from major databases including Science Direct, Chemical Abstracts, PubMed and Directory of Open Access Journals. Lichen morphology, symbiosis, diversity and bioactivities including enzyme inhibitory, antimicrobial, antifungal, antiviral, anticancer, anti-insecticidal and antioxidant actions were reviewed and summarized. Recent progress in lichens and lichen-forming fungi was discussed with emphasis on their potential to accelerate commercialization of lichen-based products. Lichens are an untapped source of biological activities of industrial importance and their potential is yet to be fully explored and utilized. Lichen-derived bioactive compounds hold great promise for biopharmaceutical applications as antimicrobial, antioxidant and cytotoxic agents and in the development of new formulations or technologies for the benefit of human life.
          Article 0 comments Cited 36 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Ecological and biotechnological aspects of lichens.

            Ilona Oksanen (2006)
            Lichens and the partners from three different kingdoms are both taxonomically and physiologically a very diverse group, which makes them interesting from both ecological and biotechnological points of view. A lichen is a mutual ecophysiological innovation in many extreme environments in which symbiosis seems to protect the partners. Lichen's ability to grow in harsh environments can be advantageous, resulting in important ecological niches, or disadvantageous when lichens occupy and cause biodeterioration of cultural monuments. Recently, new candidate compounds for drugs, UVB protection, and antifreeze proteins for frozen foods were discovered. Lichens were also found to have potential in bioplastic degradation and prevention of desertification. Nevertheless, there is still large potential for further industrial screening and research on lichen products. Due to improved culture techniques of isolated symbionts, increased knowledge of their secondary metabolism and improved methods for solubilizing lichen metabolites, the screening and activity tests can be implemented more easily today than in the past.
            Article 0 comments Cited 36 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Proteomic analysis of secreted proteins from Arabidopsis thaliana seedlings: improved recovery following removal of phenolic compounds.

              Hervé Canut, Elisabeth Jamet, Stéphane Charmont … (2005)
              Arabidopsis thaliana seedlings grown in liquid culture were used to recover proteins secreted from the whole plant. The aim was to identify apoplastic proteins that may be lost during classical extraction procedures such as preparation of cell walls. The inclusion of polyvinyl-polypyrrolidone (PVPP) in the protocol of purification of secreted proteins allowed a more efficient identification of proteins after their separation by two-dimensional gel electrophoresis (2-DE) and mass spectrometry analyses. Improvement of identification was 4-fold. It is related to an increased number of detectable peaks on mass spectra increasing the percentage of sequence coverage, and the identification confidence. The role of PVPP was to trap phenolic compounds and to prevent their unspecific interactions with proteins. These experiments resulted in the identification of 44 secreted proteins, of which 70% were not identified in previous cell wall proteomic studies. This may be due to specific gene regulation in seedlings and/or to a better access to apoplastic proteins not bound to cell walls.
              Article 0 comments Cited 23 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Mycobiology
                Journal ID (iso-abbrev): Mycobiology
                Journal ID (publisher-id): MB
                Title: Mycobiology
                Publisher: The Korean Society of Mycology
                ISSN (Print): 1229-8093
                ISSN (Electronic): 2092-9323
                Publication date (Print): June 2015
                Publication date (Electronic): 30 June 2015
                Volume: 43
                Issue: 2
                Pages: 157-162
                Affiliations
                [1 ]Institute of Biology, Scientific Educational Center, Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine.
                [2 ]Korean Lichen Research Institute, Sunchon National University, Suncheon 540-950, Korea.
                Author notes
                Corresponding author: jshur1@ 123456sunchon.ac.kr
                Article
                DOI: 10.5941/MYCO.2015.43.2.157
                PMC ID: 4505004
                SO-VID: 6499f389-72a0-42fc-8bf2-8a8ed9adad50
                Copyright © © The Korean Society of Mycology
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 15 November 2014
                Date revision received : 19 January 2015
                Date accepted : 17 May 2015
                Funding
                Funded by: National Research Foundation of Korea
                Award ID: 2013K2A4A1043370
                Award ID: 2012M3A9B8021726
                Categories
                Subject: Research Article

                ScienceOpen disciplines: Plant science & Botany
                Keywords: electrophoresis,optimization method,phenols,proteins,quinones
                Data availability:
                ScienceOpen disciplines: Plant science & Botany
                Keywords: electrophoresis, optimization method, phenols, proteins, quinones

                Comments

                Comment on this article

                scite_

                Similar content645

                See all similar

                Cited by3

                See all cited by

                Most referenced authors207

                See all reference authors