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Identification and quantification of eight flavones in root and shoot tissues of the medicinal plant Huang-qin (Scutellaria baicalensis Georgi) using high-performance liquid chromatography with diode array and mass spectrometric detection
January 2005
January 2005
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Abstract
A method of analysis of eight flavones using high-performance liquid chromatography
(HPLC)-diode array detection (DAD)-mass spectrometry (MS) in root and aerial tissues
of the medicinal plant Scutellaria baicalensis was developed. The identity of the
analytes was confirmed using retention time, UV-vis and mass spectral comparisons
to commercial standards. Both UV-vis and mass spectral patterns were characterized
for glycosylated flavones. Two additional flavone glycosides were tentatively identified
as chrysin-7-glucuronide and wogonoside, but not quantified. Greenhouse and in vitro-grown
tissues were analyzed with flavone concentrations ranges of 0.14-150 and 0.030-1.7
microg/mg for greenhouse root and shoot tissue, respectively, and 0.0068-6.4 and 0.082-1.5
microg/mg for in vitro-grown roots and shoots, respectively.