Introduction
Merkel cell carcinoma (MCC) is a rare, aggressive malignancy derived from cutaneous
neuroendocrine cells perceiving light touch.
1
MCC usually presents in elderly patients on sun-exposed areas, most commonly of the
head and neck.
2
Merkel cell polyomavirus (MCPyV) is directly involved in the pathogenesis of 80% of
MCCs in which clonal integration results in expression of 2 key antigenic oncoproteins.
3
MCPyV-negative tumors harbor many ultraviolet signature mutations with high levels
of infiltrating T lymphocytes and programmed death ligand 1 (PD-L1) expression.
4
MCC is thus an attractive target for immunotherapy because virus-positive tumors express
foreign oncoproteins, and virus-negative tumors carry ultraviolet signature mutations
providing non–self-epitopes for immune recognition.
Historically, metastatic MCC was treated with chemotherapy. Although objective response
rates (ORR) exceeded 50%, median progression-free survival (PFS) was approximately
3 months with no clear overall survival benefit.
5
Recent phase II trials of immune checkpoint blockade (ICB) in advanced MCC targeting
PD-1 with pembrolizumab or nivolumab or PD-L1 with avelumab have shown ORR of 65%
in treatment-naïve patients and 40% following chemotherapy with PFS substantially
superior to chemotherapy. However, fewer than 20% of patients achieve complete responses
(CR), and the largest trial showed 12 months PFS of 30%.
6
In March 2017, US Food and Drug Administration (FDA) approval of avelumab for advanced
MCC marked the dawn of a new era of treatment for this highly immune responsive malignancy.
Talimogene laherparepvec (TVEC) is a genetically modified herpes simplex 1 virus that
encodes human granulocyte-macrophage colony-stimulating factor to enhance dendritic
cell antigen presentation. Intratumoral injection directly lyses malignant cells and
alters the microenvironment favoring induction of systemic antitumor immunity.
7
TVEC received an indication for advanced melanoma in October 2015, making it the first
FDA-approved oncolytic viral immunotherapy. Here we describe 4 consecutive patients
treated with TVEC for regionally advanced MCC that ultimately achieved durable complete
responses to therapy all ongoing for up to greater than 27 months following TVEC initiation
with minimal toxicity. We have previously reported on cases 1 and 2
8
but have additional treatment and follow-up information.
Overview of cases
Four elderly white men underwent wide local excision of primary MCC arising in the
head or neck (Table I) obtaining negative margins where anatomically possible. Risk
factors for development of MCC included advanced age, prolonged statin use in patient
3, and immunosuppression for Crohn's disease in patient 4.
9
Serology from patients 1 and 2 was negative for antibody against MCPyV oncoprotein,
although negative predictive value is low. All patients experienced multifocal, regional
recurrence within 8 months of initial resection. TVEC therapy was selected based on
the immunogenicity of MCC, absence of detectable distant metastases, significant cardiac
comorbidity in patients 1 and 2 limiting their tolerance of potentially more toxic
treatment, and concern that ICB could exacerbate underlying autoimmune conditions
in patients 3 and 4 with Parkinson and Crohn's disease, respectively. Furthermore,
avelumab was not FDA approved for MCC until March 2017, after TVEC initiation in patients
1 and 2. TVEC was administered according to the standard dose and schedule for melanoma
into all injectable tumors. This treatment consists of an initial dose of 1 to 4 mL
of 106 pfu/mL followed 3 weeks later by doses of 1 to 4 mL of 108 pfu/mL at 2-week
intervals.
Table I
TVEC treatment outcomes
Patient
Age (yr)
No. of TVEC injected lesions
Best overall response
Durable response (Y/N)∗
PFS (mo)†
OS (mo)‡
1
87
8
CR
Y
24+
24+
2
77
4
CR
Y
19
28+
3
81
2
CR
Y
10+
10+
4
76
3
CR
Y
13+
13+
Median
—
—
—
16+
18.5+
∗
RECIST 1.1 response persisting for at least 6 months.
†
PFS calculated from first dose of TVEC.
‡
Overall survival calculated from first dose of TVEC.
Case 1
Complete resection of a right cheek MCC was followed by adjuvant radiotherapy. Biopsy
confirmed regional recurrence 7 months later with 3 dermal nodules, and positron emission
tomography/computed tomography (PET/CT) found a 9-mm hypermetabolic cutaneous nodule
in the right cheek but no evidence of nodal or hematogenous metastases. Within 3 weeks,
he had 8 palpable dermal metastases up to 1.4 cm widely distributed over the right
side of the face from the orbital rim to the jaw. With the patient's consent, TVEC
was administered on 4 occasions into all detectable metastases with toxicity limited
to mild fatigue. Nine weeks after treatment initiation, he had a complete clinical
response.
8
Serial physical examinations and PET/CTs at 3- to 4-month intervals have found no
recurrence for greater than 24 months since initiating TVEC therapy.
Case 2
An apical scalp MCC recurred 1 month after complete resection with palpable left postauricular
and level IIA/B cervical lymphadenopathy up to 3 cm with PET standardized uptake values
(SUVs) of 9.3 and 11.0, respectively, and no distant metastases. With the patient's
consent, TVEC was administered into all palpable metastases on 8 occasions with toxicity
limited to mild fatigue and transient nausea. Palpable, injectable disease resolved
within 16 weeks, and a neck CT found a partial response by RECIST 1.1 criteria with
46% reduction in index lesions with new central necrosis. PET/CT 4 months later showed
resolution of hypermetabolism and further reduction of index lesions by 62% from baseline.
8
Fifteen months after TVEC discontinuation, the patient had a newly palpable, more
anterior, left cervical node of 1.6 cm with maximum SUV of 10.7 (Fig 1). Needle biopsy
confirmed MCC, and TVEC dosing was resumed. PET/CT 3 weeks after the seventh TVEC
dose found shrinkage of the injected node to 1.1 cm with maximum SUV of 2.3 (Fig 1).
TVEC was discontinued, and repeat PET/CT in April showed radiographic CR, which is
ongoing more than 28 months after initiation of TVEC therapy.
Fig 1
Top row represents pretreatment images including a PET/CT from patient 2 depicting
the hypermetabolic left cervical node in (A) and a neck CT from patient 3 showing
the left parotid and postauricular nodules (B and C, respectively). Bottom row provides
comparable images soon after completing TVEC therapy from patient 2 (D) and patient
3 (E and F).
Case 3
A left cheek MCC recurred 5 months after complete resection with neck CT showing bulky
left parotid and left postauricular nodules 4.1 and 2.8 cm, respectively, with no
distant metastases by chest and body CT (Fig 1). Biopsy confirmed surgically incurable
MCC. With the patient's consent, TVEC was initiated, and the injected nodules transiently
enlarged after the first 3 doses consistent with pseudoprogression but subsequently
improved (Fig 2). After the seventh dose, a neck CT found the left parotid nodule
had decreased to 2.9 cm and the postauricular nodule to 1.7 cm. An eighth, final dose
of TVEC was administered followed 7 weeks later by biopsy of residual fullness in
the left parotid region, which found fibroconnective tissue and plasma cells with
no malignancy. Neck CT found resolution of the postauricular nodule to linear scarring
(Fig 1). Toxicity consisted of mild, transient flu-like symptoms. Physical examinations
and serial CT scans at 3-month intervals have shown no recurrence for more than 10 months
after TVEC initiation.
Fig 2
Photographs from patient 3 obtained immediately before TVEC initiation (A), with pseudoprogression
after the third dose (B), and after dose 6 of 8 with marked improvement (C).
Case 4
A patient with Crohn's disease treated with infliximab converted to vedolizumab, a
gut-selective anti-inflammatory agent, had a right anterior neck MCC. Six months after
wide local incision, MCC recurred with 2 palpable, dermal nodules along the scar.
CT scans of the chest and body found no distant spread. Before resection could be
performed, a third palpable metastasis developed in the right neck. TVEC was initiated
with the patient's consent into 3 tumor nodules measuring up to 1.2 cm. After 5 doses,
a clinical CR was achieved. Toxicity was minimal with no activation of Crohn's disease,
and the patient has maintained a CR with serial physical examinations and PET/CTs
for more than 13 months since TVEC initiation.
Discussion
ICB of PD-1/PD-L1 interactions is the most effective approved therapy for surgically
incurable MCC. However, CRs are rare, and most patients progress in less than a year.
Here we report promising results with TVEC in 4 consecutive patients with recurrent
regionally advanced MCC. Durable CRs were achieved in all 4 patients with a median
PFS of greater than 16 months and no serious adverse events (Table I). In addition
to complete regional response of injected tumors, TVEC has also prevented outgrowth
of distant metastases in these high-risk individuals.
Our observations parallel the clinical trial experience with TVEC in melanoma showing
greatest efficacy in stage IIIB/C patients with regionally advanced disease.
10
Interim analysis of a randomized phase II trial of TVEC neoadjuvant treatment plus
surgery versus surgery alone for resectable stage IIIB to IVM1a melanoma found a pathologic
CR rate of 21% and an improved R0 resection rate from 41% to 56%.
11
Phase II trials are underway examining TVEC monotherapy in surgically incurable MCC,
basal cell carcinoma, and squamous cell skin cancer and TVEC with or without radiotherapy
for regionally advanced MCC or melanoma (Table II). Until data are available from
these prospective studies, our observations provide compelling evidence that TVEC
has important clinical activity in this rare malignancy and suggests a potential role
for TVEC in marginally resectable MCC worthy of prospective evaluation.
Table II
Current trials of TVEC in cutaneous malignancies
Treatment trial design
Cancer types
Indication
Clinical Trials.gov
Registry no.
TVEC monotherapy
Phase IIMCC, SCC, and BCC
Locally advanced
NCT03458117
TVEC ± radiotherapy
Phase IIMCC and melanoma
Locally advanced
NCT02819843
TVEC + nivolumab
Phase IIMCC, SCC, and BCC
Locally advanced or widely metastatic
NCT02978625
TVEC + pembrolizumab
Phase Ib/IIImelanoma
Locally advanced or widely metastatic
NCT02263508
BCC, Basal cell carcinoma; SCC, squamous cell carcinoma.
Although single-agent TVEC has limited utility in stage IV melanoma with lung or visceral
metastases, combining it with ipilimumab CTLA-4 ICB appeared to enhance the ORR and
PFS compared with monotherapy with either agent.
12
Similarly, the phase Ib portion of a phase Ib/III trial combining TVEC with pembrolizumab
PD-1 ICB in advanced melanoma reported objective responses in 62% of patients with
a CR rate of 33%, superior to either single agent.
13
Based on these observations, a basket trial is evaluating the combination of TVEC
with nivolumab in advanced or refractory nonmelanoma skin cancers including MCC (Table
II).
The landscape of available cancer immunotherapeutics is evolving so rapidly that prospective
evaluation of monotherapies and combinations in a malignancy as rare as MCC may not
allow timely access to treatment. The combination of retrospective case series as
provided here and extrapolation from similar, immune-responsive, more prevalent skin
malignancies like melanoma may allow clinical implementation of important treatment
advances years before prospective data can be obtained.