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[Determination of serum iron; a comparison of two methods: Teepol/dithionite/bathophenanthroline versus guanidine/ascorbic acid/Ferrozine (author's transl)].
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Abstract
Two spectrophotometric methods for the assay of serum iron without deproteinisation are compared: 1. Liberation of iron by a detergent (Teepol SHELL), reduction by dithionite, chelation by bathopehanthroline disulfonate; 2. Liberation of iron by 6 mol/l guanidine, reduction by ascorbic acid, chelation by 3-(2-pyridyl)-5,6-bis-(4-phenyl sulfonic acid)-1,2,4-triaxine (Ferrozine).