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Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments
February 2001
February 2001
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Abstract
We used chromatography modeling software to assist in HPLC method development, with
the goal of enhancing separations through the exclusive use of gradient time and column
temperature. We surveyed nine stationary phases for their utility in pigment purification
and natural sample analysis. For purification, a complex algal matrix was separated
on an efficient monomeric column, from which partially purified fractions were collected
and purified on polymeric columns that exaggerated resolution between pigments of
interest. Additionally, we feature an HPLC method that is simple, fast, demonstrates
excellent transferability and is ideal for quantitative analysis of pigments in dilute
natural water samples.