Ibuprofen inhibits granulocyte responses to inflammatory mediators : A proposed mechanism for reduction of experimental myocardial infarct size

An in vitro technique is described for assessing the chemotactic activity of soluble substances on motile cells. Antibody-antigen mixtures when incubated (37°C) in medium containing fresh (i.e. non-inactivated) normal rabbit serum exert a strong chemotactic effect on rabbit polymorphonuclear leucocytes. Results are described which indicate that, when antibody-antigen complexes are incubated (37°C) in fresh serum, a heat-stable (56°C) substance (or substances) is produced which acts directly as a chemotactic stimulus on the polymorphs. This heat-stable chemotactic substance is not produced when antibody-antigen complexes are incubated in serum which has been heated at 56°C for 30 minutes.

Polymorphonuclear leukocytes infiltrate tissues in response to an inflammatory stimulus to remove invading microorganisms and cell debris. We present eivdence that these scaveging cells have another, more sophisticated role in that they are involved in the control of fluid efflux through the blood vessel wall which leads to tissue oedema.