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      Estudo da atividade metanogênica específica de diferentes lodos anaeróbios Translated title: Study of specific methanogenic activity of different anaerobic sludges

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          Abstract

          RESUMO O estudo da atividade metanogênica específica (AME) constitui uma importante ferramenta para o controle operacional de reatores anaeróbios, pois apresenta parâmetros de monitoramento da eficiência e estabilidade de um reator biológico. Nesse sentido, o presente trabalho visou determinar a AME de lodos provenientes de reatores anaeróbios de fluxo ascendente (UASB - upflow anaerobic slugde blanket) de duas indústrias cervejeiras (lodos A e B), uma de processamento de grãos (lodo C), uma leiteira (lodo D) e uma de processamento de couro (lodo E). Os ensaios foram conduzidos em batelada, em ambiente aclimatado a 35°C, utilizando um equipamento para monitorar a geração de biogás em função do tempo de digestão. Também foi realizada a qualificação do gás metano presente no biogás gerado pelos diferentes lodos. O maior valor de AME obtido e a maior porcentagem do gás metano produzido, para uma concentração de biomassa de 5,0 gSTV.L-1 e 4,0 gDQO.L-1 de glicose, foram para o lodo B: 0,36 gDQO-CH4.gSTV-1.d-1 e 58,9%, respectivamente.

          Translated abstract

          ABSTRACT The study of Specific Methanogenic Activity (SMA) is an important tool for operational control of anaerobic reactors, as it presents parameters to monitor the efficiency and stability of a biological reactor. For this reason, the present article aimed to determine the SMA of sludges from UASB reactors, of two different brewing industries (sludges A and B), a grain processing industry (sludge C), a dairy industry (sludge D), and a leather processing industry (sludge E). Assays were performed in batch, at an acclimatized environment (35°C), using a device equipment to monitor the generation of biogas due to digestion time. Also, the quantity of methane gas was measured in the biogas generated by the different sludges. The best SMA value obtained and the greater percentage of methane gas generated, with a biomass concentration of 5.0 gSTV.L-1, and a glucose concentration of 4.0 gCOD.L-1, were for sludge B, with 0.36 gCOD-CH4.gSTV-1.d-1 and 58.9%, respectively.

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          Metodologias para determinação da atividade metanogênica específica (AME) em lodos anaeróbios

          Esse artigo apresenta uma revisão sobre os diferentes protocolos para a determinação da atividade metanogênica específica (AME) de lodos anaeróbios. Os protocolos propostos se diferem tanto em relação aos procedimentos adotados para a incubação do lodo, quanto ao método utilizado para quantificação do metano produzido durante o teste. São discutidos os princípios dos métodos manométricos e volumétricos, e descritos brevemente os protocolos de incubação do lodo, de medição do metano e o procedimento para cálculo da AME obtida pelos métodos mais utilizados pela comunidade científica nacional.
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            Methanogenic activities in anaerobic membrane bioreactors (AnMBR) treating synthetic municipal wastewater

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              Microbial analysis in biogas reactors suffering by foaming incidents.

              Foam formation can lead to total failure of digestion process in biogas plants. In the present study, possible correlation between foaming and the presence of specific microorganisms in biogas reactors was elucidated. The microbial ecology of continuous fed digesters overloaded with proteins, lipids and carbohydrates before and after foaming incidents was characterized using 16S rRNA gene sequencing. Moreover, the microbial diversity between the liquid and foaming layer was assessed. A number of genera that are known to produce biosurfactants, contain mycolic acid in their cell wall, or decrease the surface tension of the media, increased their relative abundance after foam formation. Finally, a microorganism similar to widely known foaming bacteria (Nocardia and Desulfotomaculum) was found to increase its relative abundance in all reactors once foam was observed, regardless of the used substrate. These findings suggest that foaming and specific microorganisms might have direct association which requires to be further investigated.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                esa
                Engenharia Sanitaria e Ambiental
                Eng. Sanit. Ambient.
                Associação Brasileira de Engenharia Sanitária e Ambiental - ABES (Rio de Janeiro, RJ, Brazil )
                1413-4152
                1809-4457
                December 2019
                : 24
                : 6
                : 1139-1146
                Affiliations
                [1] Chapecó Santa Catarina orgnameUniversidade Comunitária da Região de Chapecó Brazil
                [2] Chapecó SC orgnameGenética Tecnologias Ambientais Brasil
                Article
                S1413-41522019000601139 S1413-4152(19)02400601139
                10.1590/s1413-41522019140730
                736c5921-9888-4bfc-8afc-bb467d8554f1

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 18 September 2014
                : 19 July 2019
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 25, Pages: 8
                Product

                SciELO Brazil

                Categories
                Artigo Técnico

                metano,reator anaeróbio de fluxo ascendente (UASB),upflow anaerobic sludge blanket,atividade metanogênica específica,specific methanogenic activity,methane,lodos anaeróbios,anaerobic sludge

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