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      Simultaneous determination of six isoflavonoids in commercial Radix Astragali by HPLC-UV.

      Fitoterapia
      Astragalus Plant, Chromatography, High Pressure Liquid, Humans, Isoflavones, chemistry, Phytotherapy, Spectrophotometry, Ultraviolet

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          Abstract

          A HPLC-UV method for the quantification of six major isoflavonoids, calycosin 7-O-beta-D-glucoside (1), formononetin 7-O-beta-D-glucoside (2), (6alphaR, 11alphaR) 3-hydroxy-9,10-dimethoxypterocarpan-3-O-beta-D-glucoside (3), 7,2'-dihydroxy-3',4'-dimethoxyisoflavan-7-O-beta-D-glucoside (4), calycosin (5) and formononetin (6), in Radix Astragali (Huangqi) was developed and validated. The method was proven to be sensitive, specific, accurate and precise, as well as effective and easy.

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          Author and article information

          Journal
          15752625
          10.1016/j.fitote.2004.11.006

          Chemistry
          Astragalus Plant,Chromatography, High Pressure Liquid,Humans,Isoflavones,chemistry,Phytotherapy,Spectrophotometry, Ultraviolet

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