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      Hydropriming changes temperature thresholds for seed germination of tree species from the Caatinga, a Brazilian tropical dry forest Translated title: Hidropriming altera limiares de temperatura para germinação de sementes de espécies arbóreas da Caatinga, uma floresta tropical seca brasileira

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          Abstract

          Abstract: This study evaluates the effects of hydration and dehydration cycles (HD cycles) on seed germination of four Caatinga tree species (Anadenanthera colubrina var. cebil, Enterolobium contortisiliquum, Pityrocarpa moniliformis and Pterogyne nitens) subjected to different temperatures and determine the thermal upper and lower limits of germination. For this, seeds were subjected to 0, 1, 2 and 3 HD cycles and set to germinate at temperatures of 5, 10, 15, 25, 35, 40 and 45 °C. Germinability and t50 were calculated and the differences of these parameters were compared by two-way ANOVA. In addition, germination rate (GR = 1/t50), as well as ceiling (Tc), optimum (To) and base (Tb) temperatures were calculated to estimate the limits below or above which the seeds fail to germinate. We observed that the more HD cycles, the better the seed responds, especially at extreme temperatures. Seeds of all studied species showed To between 25 and 35 °C, Tb < 10 °C, and Tc > 40 °C. The slope of regression curves for germination rate showed that submitting seeds to HD cycles expands its thermal tolerance range, revealing that this seed pre-treatment can be efficient for species as it increases their tolerance to thermal stress.

          Translated abstract

          Resumo: Este estudo avaliou os efeitos dos ciclos de hidratação e desidratação (ciclos de HD) na germinação de sementes de quatro espécies arbóreas da Caatinga (Anadenanthera colubrina var. cebil, Enterolobium contortisiliquum, Pityrocarpa moniliformis and Pterogyne nitens) submetidas a diferentes temperaturas, determinando os limites térmicos para germinação. As sementes foram submetidas a 0, 1, 2 e 3 ciclos de HD e colocadas para germinar nas temperaturas de 5, 10, 15, 25, 35, 35, 40 e 45 °C. Germinabilidade e t50 foram calculados e as diferenças foram comparadas pela ANOVA Dois Fatores. Além disso, a taxa de germinação (GR = 1/t50), bem como temperaturas teto (Tc), ótima (To) e base (Tb) foram calculadas para estimar os limites abaixo ou acima dos quais as sementes não germinam. Foi observado que quanto mais ciclos de HD, melhor é a resposta da semente, principalmente em temperaturas extremas. Sementes de todas as espécies apresentaram To entre 25 e 35 °C, Tb < 10 °C e Tc > 40 °C. A inclinação das curvas de regressão da taxa de germinação mostrou que os ciclos de HD expandem faixa de tolerância térmica das sementes, revelando que esse pré-tratamento pode ser eficiente para as espécies, aumentando sua tolerância ao estresse térmico.

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          Most cited references36

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          Genes commonly regulated by water-deficit stress in Arabidopsis thaliana.

          Cellular water-deficit stress triggers many changes in gene expression which can be used to define the response of a plant to an environmental condition. Microarray technology permits the study of expression patterns of thousands of genes simultaneously, permitting a comprehensive understanding of the types and quantities of RNAs that are present in a cell in response to water-deficit stress. The expression of specific genes was compared in three different experiments designed to understand changes in gene expression in response to water-deficit stress. Surprisingly, there was a relatively small set of genes that were commonly induced or repressed. There were 27 genes commonly induced and three commonly repressed; 1.4% and 0.2% of the genes analysed in common to all three experiments. The induced genes fell into six different functional categories: metabolism, transport, signalling, transcription, hydrophilic proteins, and unknown. The three commonly repressed genes indicated that repression of gene expression supported a frequently observed response to water-deficit stress, decreased growth. A more detailed analysis of genes involved in cell wall metabolism, indicated that there was a global decrease in expression of genes that promote cell expansion.
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            Priming memory invokes seed stress-tolerance

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              Thermal Hardening: A New Seed Vigor Enhancement Tool in Rice

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                Author and article information

                Journal
                jss
                Journal of Seed Science
                J. Seed Sci.
                ABRATES - Associação Brasileira de Tecnologia de Sementes (Londrina, PR, Brazil )
                2317-1537
                2317-1545
                2021
                : 43
                : e202143004
                Affiliations
                [1] Itabaiana orgnameUniversidade Federal de Sergipe orgdiv1Departamento de Biociências orgdiv2Laboratório de Fisiologia de Sementes Brazil
                [3] Petrolina orgnameEmpresa Brasileira de Pesquisa Agropecuária orgdiv1Centro de Pesquisa Agropecuária do Trópico Semiárido orgdiv2Laboratório de Análise de Sementes Brazil
                [2] São Cristóvão orgnameUniversidade Federal de Sergipe Brazil
                Article
                S2317-15372021000100101 S2317-1537(21)04300000101
                10.1590/2317-1545v43238649
                75af46d0-9238-4d9c-8df3-b854bfaf0723

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 25 May 2020
                : 12 January 2021
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 36, Pages: 0
                Product

                SciELO Brazil

                Self URI: Full text available only in PDF format (EN)
                Categories
                Article

                thermal stress,tolerance,HD cycles,modelagem,estresse térmico,tolerância,ciclos de HD,modeling

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