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      Candida bombicola as a platform organism for the production of tailor-made biomolecules.

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          Abstract

          The yeast Candida bombicola is capable of producing high amounts (400 g/L) of the biosurfactant sophorolipids. The genetic makeup of this industrially important yeast has recently been uncovered and molecular manipulation techniques have been developed. Hence, all tools for the development of new bioprocesses with C. bombicola are now available. As a proof of concept, the production of two totally different molecules was aimed for: the bioplastic polyhydroxyalkanoate (PHA) and a new-to-nature cellobioselipid-biosurfactant. Integration of the new functionalities at genomic loci necessary for sophorolipid production safeguards the new biomolecules from sophorolipid contamination, while taking advantage of the regulation of the sophorolipid gene cluster. A maximum yield of 2.0% wt/dwt PHA was obtained; furthermore, this is the first time cellobioselipid synthesis by a non-natural producer is reported. We here provided proof of concept that C. bombicola can be transformed into a platform organism for the production of tailor-made biomolecules.

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          Author and article information

          Journal
          Biotechnol. Bioeng.
          Biotechnology and bioengineering
          Wiley
          1097-0290
          0006-3592
          Sep 2013
          : 110
          : 9
          Affiliations
          [1 ] Faculty of Bioscience Engineering, Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Ghent University, Coupure Links 653, Ghent, Belgium.
          Article
          10.1002/bit.24895
          23475585
          77058c4d-27b9-4b29-8a86-382eb4af4b2e
          History

          beta-oxidation,biosurfactants,cellobioselipids,tailor-made biomolecules,Candida bombicola,PHA

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