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      Scaffold-free vascular tissue engineering using bioprinting

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      Biomaterials
      Elsevier BV

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          Abstract

          Current limitations of exogenous scaffolds or extracellular matrix based materials have underlined the need for alternative tissue-engineering solutions. Scaffolds may elicit adverse host responses and interfere with direct cell-cell interaction, as well as assembly and alignment of cell-produced ECM. Thus, fabrication techniques for production of scaffold-free engineered tissue constructs have recently emerged. Here we report on a fully biological self-assembly approach, which we implement through a rapid prototyping bioprinting method for scaffold-free small diameter vascular reconstruction. Various vascular cell types, including smooth muscle cells and fibroblasts, were aggregated into discrete units, either multicellular spheroids or cylinders of controllable diameter (300-500 microm). These were printed layer-by-layer concomitantly with agarose rods, used here as a molding template. The post-printing fusion of the discrete units resulted in single- and double-layered small diameter vascular tubes (OD ranging from 0.9 to 2.5mm). A unique aspect of the method is the ability to engineer vessels of distinct shapes and hierarchical trees that combine tubes of distinct diameters. The technique is quick and easily scalable.

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          Author and article information

          Journal
          Biomaterials
          Biomaterials
          Elsevier BV
          01429612
          October 2009
          October 2009
          : 30
          : 30
          : 5910-5917
          Article
          10.1016/j.biomaterials.2009.06.034
          2748110
          19664819
          7817ccd0-5ec9-4c5e-9af4-50c39be51697
          © 2009

          https://www.elsevier.com/tdm/userlicense/1.0/

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