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      Differences in the antimicrobial susceptibility profiles of Moraxella bovis, M. bovoculi and M. ovis

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          Abstract

          The aim of this study was to determine the differences in the antimicrobial susceptibility profiles of Moraxella bovis, M. bovoculi and M. ovis. Thirty-two strains of Moraxella spp. isolated from cattle and sheep with infectious keratoconjunctivitis were tested via broth microdilution method to determine their susceptibility to ampicillin, cefoperazone, ceftiofur, cloxacillin, enrofloxacin, florfenicol, gentamicin, neomycin, oxytetracycline and penicillin. The results demonstrated that Moraxella spp. strains could be considered sensitive for most of the antimicrobials tested in this study, but differences between the antimicrobial susceptibility profiles of these three Moraxella species were found. M. bovis might differ from other species due to the higher MIC and MBC values it presented.

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          Molecular Cloning : A Laboratory Manual

          Joseph Sambrook, David William Russell (2001)
          <p>The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.<br>In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.<br>Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques.<br>The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.<br>These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.<br>The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions.<br>The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.<br>As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved. </p>
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            Biochemical Tests for Identification of Medical Bacterial

            J.F. MacFaddin, J. F. MacFaddin, J. MacFaddin … (2000)
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              Moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis.

              Phillip Q Spinks, John Angelos, Kevin W. George … (2007)
              Eighteen isolates of a Gram-negative coccus (strain 237(T)) were cultured from the eyes of dairy and beef calves affected with infectious bovine keratoconjunctivitis (IBK; 'pinkeye') in northern California, USA, during summer 2002. These isolates had near full-length (1397 bp) 16S rRNA gene sequences that clustered into three groups with 99.9 % sequence similarity. On the basis of 16S rRNA gene sequence, the isolates were most closely associated with Moraxella bovis and Moraxella ovis in clade I of the classical moraxellae. Biochemically, the novel isolates could be distinguished from the other members of the genus Moraxella isolated from animals on the basis of phenylalanine deaminase activity. The results of partial sequence analysis of six housekeeping genes, the 16S-23S rRNA gene interspacer region and partial 23S rRNA gene provide strong support for the inclusion of these isolates in a novel taxon, for which the name Moraxella bovoculi sp. nov. is proposed. The type strain is strain 237(T) (=ATCC BAA-1259(T)=CCUG 52049(T)).
              Article 0 comments Cited 27 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Braz J Microbiol
                Journal ID (iso-abbrev): Braz. J. Microbiol
                Journal ID (publisher-id): bjm
                Title: Brazilian Journal of Microbiology
                Publisher: Sociedade Brasileira de Microbiologia
                ISSN (Print): 1517-8382
                ISSN (Electronic): 1678-4405
                Publication date (Electronic): 01 June 2015
                Publication date Collection: June 2015
                Volume: 46
                Issue: 2
                Pages: 545-549
                Affiliations
                [1 ]Universidade Federal de Santa Maria, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
                [2 ]Universidade Federal de Santa Maria, Departamento de Zootecnia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil, Departamento de Zootecnia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
                Author notes
                Send correspondence to A.C. Vargas. Laboratório de Bacteriologia, Universidade Federal de Santa Maria, Campus UFSM, prédio 44, sala 5137, 97105-900 Santa Maria, RS, Brazil. E-mail: agueda.vargas@ 123456gmail.com .

                Associate Editor: Odir Antonio Dellagostin

                Article
                DOI: 10.1590/S1517-838246220140058
                PMC ID: 4507549
                SO-VID: 789a712e-e152-4503-913e-01eb5602b347
                Copyright © Copyright © 2015, Sociedade Brasileira de Microbiologia
                License:

                All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.

                History
                Date received : 22 January 2014
                Date accepted : 15 August 2014
                Page count
                Figures: 0, Tables: 2, Equations: 0, References: 20, Pages: 5
                Categories
                Subject: Veterinary Microbiology

                Keywords: bacterial resistance,broth microdilution,eye disease
                Data availability:
                Keywords: bacterial resistance, broth microdilution, eye disease

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