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      Prolactin Control of Growth and Prolactin Autoregulation in Cultured Human Pituitary Cells


      Hormone Research in Paediatrics

      S. Karger AG

      Human pituitary, Prolactin, Growth, Serum-free medium, Cell culture

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          A human pituitary cell line (18-54, SF) grows in serum-free medium and secretes prolactin (PRL). Autoregulation of pituitary cell growth and PRL production by exogenously supplied ovine PRL (oPRL) was investigated. Human PRL (hPRL) and oPRL stimulated pituitary cell growth up to 92% and 85%, respectively, at hPRL and oPRL additions of 100-1,000 ng/ml. Short-term (1 h) incubation of the cells with oPRL decreased hPRL secretion from the cells by 72% at 10 ng/ml addition. Intracellular hPRL was stimulated under the same conditions by 50-275% at oPRL concentrations of 10-1,000 ng/ml. Long-term (10 days) incubation of the cells with oPRL had no significant effect on extracellular or intracellular hPRL production. These data suggest that the pituitary gland can serve as a primary feedback site and that PRL can autoregulate its own production as well as affect the growth of pituitary cells.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          26 November 2008
          : 21
          : 4
          : 240-245
          Departments of Biological Sciences and Biochemistry, University of Missouri, Columbia, Mo., USA, Department of Biological Sciences, Hunter College of CUNY, New York, N.Y., USA
          180056 Horm Res 1985;21:240–245
          © 1985 S. Karger AG, Basel

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          Page count
          Pages: 6


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