Sex and stress: Men and women show different cortisol responses to psychological stress induced by the Trier social stress test and the Iowa singing social stress test : Sex and the Trier and Iowa Singing Social Stress Tests

Abstract

<p class="first" id="P1">Acute psychological stress affects each of us in our daily lives and is increasingly a topic of discussion for its role in mental illness, aging, cognition, and overall health. Better understanding of how such stress affects the body and mind could contribute to the development of more effective clinical interventions and prevention practices. Over the past three decades, the Trier Social Stress Test (TSST) has been widely used to induce acute stress in a laboratory setting based on the principles of social evaluative threat - namely, a judged speech-making task. A comparable alternative task may expand options for examining acute stress in a controlled laboratory setting. Here, we used a within-subjects design to examine healthy adult participants’ ( <i>n</i>=20 men, <i>n</i>=20 women) subjective stress and salivary cortisol responses to the standard TSST (involving public speaking and math) and the newly created Iowa Singing Social Stress Test (I-SSST). The I-SSST is similar to the TSST, but with a new twist: public <i>singing</i>. Results indicate that men and women reported similarly high levels of subjective stress in response to both tasks. However, men and women demonstrated different cortisol responses: men showed a robust response to both tasks, and women displayed a smaller response. These findings are consistent with previous literature, and further underscore the importance of examining possible sex differences throughout various phases of research, including design, analysis, and interpretation of results. Furthermore, this nascent examination of the I-SSST suggests a possible alternative for inducing stress in the laboratory. </p><p id="P2"> <div class="figure-container so-text-align-c"> <img alt="" class="figure" src="/document_file/5febc598-59eb-4932-970c-b9a9cc8b8c36/PubMedCentral/image/nihms801226f5.jpg"/> </div> </p><p id="P3">Mean change in salivary cortisol (nmol/L) from baseline over time after the Iowa Singing Social Stress Test (I-SSST) for women (gray circles) and men (black squares; n = 36). Error bars indicate standard error of the mean. The figure presents untransformed change values, while the reported analysis in text used natural log transformed data. </p><p id="P4">The Iowa Singing Social Stress Test - a novel, musical stress task - elicited responses similar to those induced by the Trier Social Stress Test. We found a robust sex difference in cortisol response yet no sex difference in subjective stress. Our results suggest an alternative acute stress induction method. </p>