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      Impact of conjugation strategies for targeting of antibodies in gold nanoparticles for ultrasensitive detection of 17β-estradiol

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          Abstract

          Antibody-coated nanoparticles have recently attracted considerable attention, with the focus falling on diagnostics. Nevertheless, controlled antibody bioconjugation remains a challenge. Here, we present two strategies of bioconjugation with the aim of evaluating the best approach for the coupling of antibodies on the surface of nanomaterials in an oriented way. We employed electrostatic interaction (physical adsorption) and covalent conjugation in the orientation of antibodies on the metallic surface as coupling methods, and their influence on the detection of 17β-estradiol was addressed with localized surface plasmon resonance. The understanding of these mechanisms is fundamental for the development of reproducible inorganic bioconjugates with oriented surface as well sensibility of immunoassays.

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          Ligand-targeted therapeutics in anticancer therapy.

          Theresa Allen (2002)
          Cytotoxic chemotherapy or radiotherapy of cancer is limited by serious, sometimes life-threatening, side effects that arise from toxicities to sensitive normal cells because the therapies are not selective for malignant cells. So how can selectivity be improved? One strategy is to couple the therapeutics to antibodies or other ligands that recognize tumour-associated antigens. This increases the exposure of the malignant cells, and reduces the exposure of normal cells, to the ligand-targeted therapeutics.
          Article 0 comments Cited 303 times – based on 0 reviews     Review now
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            Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles.

            R A Sperling, W. Parak (2010)
            Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y(2)O(3)), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.
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              DNA assembly of nanoparticle superstructures for controlled biological delivery and elimination

              Leo Y.T. Chou, Kyryl Zagorovsky, Warren C.W. Chan (2014)
              The assembly of nanomaterials using DNA can produce complex nanostructures, but the biological applications of these structures remain unexplored. Here we describe the use of DNA to control the biological delivery and elimination of inorganic nanoparticles by organizing them into colloidal superstructures. The individual nanoparticles serve as building blocks, whose size, surface chemistry, and assembly architecture dictate overall superstructure design. These superstructures interact with cells and tissues as a function of their design, but subsequently degrade into building blocks that can escape biological sequestration. We demonstrate that this strategy reduces nanoparticle retention by macrophages and improves their in vivo tumour accumulation and whole-body elimination. Superstructures can be further functionalized to carry and protect imaging or therapeutic agents against enzymatic degradation. These results suggest a new strategy to engineer nanostructure interactions with biological systems and highlight new directions in the design of biodegradable and multifunctional nanomedicine.
              Article 0 comments Cited 93 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Marco Cesar Cunegundes Guimarães: jairo.oliveira@ufes.br
                Journal
                Journal ID (nlm-ta): Sci Rep
                Journal ID (iso-abbrev): Sci Rep
                Title: Scientific Reports
                Publisher: Nature Publishing Group UK (London )
                ISSN (Electronic): 2045-2322
                Publication date (Electronic): 25 September 2019
                Publication date PMC-release: 25 September 2019
                Publication date Collection: 2019
                Volume: 9
                Electronic Location Identifier: 13859
                Affiliations
                [1 ]ISNI 0000 0001 2167 4168, GRID grid.412371.2, Federal University of Espirito Santo, ; Av Marechal Campos1468, Vitoria, ES 29.040-090 Brazil
                [2 ]Federal Institute of Espírito Santo, km 6.5 ES 010, Serra, ES 29173-087 Brazil
                [3 ]ISNI 0000 0001 2167 4168, GRID grid.412371.2, Federal University of Espirito Santo, ; Av. Fernando Ferrari, Vitoria, ES, 29075-910 Brazil
                Article
                Publisher ID: 50424
                DOI: 10.1038/s41598-019-50424-5
                PMC ID: 6761283
                PubMed ID: 31554912
                SO-VID: 7e7d272b-260e-4531-9755-a39c38c21b67
                Copyright © © The Author(s) 2019
                License:

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

                History
                Date received : 23 July 2019
                Date accepted : 12 September 2019
                Categories
                Subject: Article
                Custom metadata
                issue-copyright-statement © The Author(s) 2019

                ScienceOpen disciplines: Uncategorized
                Keywords: biosensors,nanoparticles
                Data availability:
                ScienceOpen disciplines: Uncategorized
                Keywords: biosensors, nanoparticles

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